C57BL/6JCya-Pkn1em1/Cya
Common Name:
Pkn1-KO
Product ID:
S-KO-19033
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Pkn1-KO
Strain ID
KOCMP-320795-Pkn1-B6J-VB
Gene Name
Product ID
S-KO-19033
Gene Alias
DBK; F730027O18Rik; PAK1; PRK1; Pkn; Prkcl1; Stk3
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
8
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Pkn1em1/Cya mice (Catalog S-KO-19033) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000144258
NCBI RefSeq
NM_001199593
Target Region
Exon 2
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
Protein kinase N1 (PKN1), a serine/threonine kinase, has been shown to play crucial roles in multiple biological processes. It contributes to Glut4 translocation and glucose transport, regulating adipocyte differentiation and glucose metabolism [1]. PKN1 is also involved in the regulation of synaptic maturation in the brain, and its functions are associated with pathways like AKT and the regulation of transcription factors such as NeuroD2 [2,3].
Pkn1 knockout mouse models have revealed significant insights. In the hippocampus, Pkn1 knockout led to elevated phospho-AKT and NeuroD2 levels, enhancing the expression of the AMPAR subunit GluA1, which may be related to neurological disorders [2]. In an in vitro model of cerebellar hypoxic-ischemic encephalopathy, Pkn1 knockout in cerebellar granule cells showed higher AKT phosphorylation, reduced caspase-3 activation, and improved survival, indicating a neurodegenerative role of PKN1 [3]. In retinal cell type formation, Pkn1 knockout led to changes in the number of certain cell types, suggesting its importance in retinal development [4]. In mouse embryonic fibroblasts, PKN1 kinase-deficient cells showed delayed aggregate formation and abnormal spheroid compaction [5]. In aged PKN1 kinase-negative knock-in mice, there was splenomegaly and leukopenia without obvious autoimmune-like phenotypes, highlighting potential kinase-independent functions of PKN1 [6].
In conclusion, PKN1 is a key regulator in various biological processes, including adipocyte function, synaptic maturation, and cell survival in the nervous system. The use of Pkn1 knockout and kinase-negative knock-in mouse models has provided valuable insights into its roles in diseases such as insulin resistance, neurodegenerative conditions, and potential hematological disorders. These findings may offer new therapeutic approaches for related diseases.
References:
1. Herrerías-González, Fernando, Yeramian, Andrée, Baena-Fustegueras, Juan Antonio, Mora, Conchi, Lecube, Albert. 2023. PKN1 Kinase: A Key Player in Adipocyte Differentiation and Glucose Metabolism. In Nutrients, 15, . doi:10.3390/nu15102414. https://pubmed.ncbi.nlm.nih.gov/37242297/
2. Safari, Motahareh Solina, Obexer, Dido, Baier-Bitterlich, Gabriele, Zur Nedden, Stephanie. 2021. PKN1 Is a Novel Regulator of Hippocampal GluA1 Levels. In Frontiers in synaptic neuroscience, 13, 640495. doi:10.3389/fnsyn.2021.640495. https://pubmed.ncbi.nlm.nih.gov/33613259/
3. Zur Nedden, Stephanie, Safari, Motahareh Solina, Fresser, Friedrich, Baier, Gottfried, Baier-Bitterlich, Gabriele. 2023. PKN1 Exerts Neurodegenerative Effects in an In Vitro Model of Cerebellar Hypoxic-Ischemic Encephalopathy via Inhibition of AKT/GSK3β Signaling. In Biomolecules, 13, . doi:10.3390/biom13111599. https://pubmed.ncbi.nlm.nih.gov/38002281/
4. Brunner, Magdalena, Lang, Luisa, Künkel, Louisa, Baier-Bitterlich, Gabriele, Zur Nedden, Stephanie. 2024. Role of PKN1 in Retinal Cell Type Formation. In International journal of molecular sciences, 25, . doi:10.3390/ijms25052848. https://pubmed.ncbi.nlm.nih.gov/38474095/
5. Mehruba, Mona, Siddique, Salman Mahmud, Mukai, Hideyuki. 2019. PKN1 controls the aggregation, spheroid formation, and viability of mouse embryonic fibroblasts in suspension culture. In Biochemical and biophysical research communications, 523, 398-404. doi:10.1016/j.bbrc.2019.12.069. https://pubmed.ncbi.nlm.nih.gov/31870546/
6. Siddique, Salman Mahmud, Kubouchi, Koji, Shinmichi, Yuka, Itoh, Tomoo, Mukai, Hideyuki. 2019. PKN1 kinase-negative knock-in mice develop splenomegaly and leukopenia at advanced age without obvious autoimmune-like phenotypes. In Scientific reports, 9, 13977. doi:10.1038/s41598-019-50419-2. https://pubmed.ncbi.nlm.nih.gov/31562379/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen