C57BL/6JCya-Fbh1em1/Cya
Common Name
Fbh1-KO
Product ID
S-KO-19131
Backgroud
C57BL/6JCya
Strain ID
KOCMP-50755-Fbh1-B6J-VC
When using this mouse strain in a publication, please cite “Fbh1-KO Mouse (Catalog S-KO-19131) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Fbh1-KO
Strain ID
KOCMP-50755-Fbh1-B6J-VC
Gene Name
Product ID
S-KO-19131
Gene Alias
Fbx18, Fbxo18
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 2
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000071564
NCBI RefSeq
NM_015792
Target Region
Exon 4~6
Size of Effective Region
~2.5 kb
Overview of Gene Research
Fbh1, or F-box DNA helicase 1, is a member of the UvrD family of DNA helicases. It plays crucial roles in the DNA damage response and the regulation of cell responses to replicative stress [3,4,6]. FBH1 is involved in pathways related to homologous recombination, replication fork processing, and checkpoint control, which are essential for maintaining genome integrity [2,3,7].
FBH1-deficient cells show a reduction in ssDNA and double-strand break signaling, indicating its requirement for the induction of replication stress response in cells treated with WEE1 inhibitors. Despite this defect, FBH1-deficiency sensitizes cells to WEE1 inhibition by increasing mitotic catastrophe, suggesting that loss of FBH1 leads to replication-associated damage that needs the WEE1-dependent G2 checkpoint for repair [1,5]. Also, FBH1 depletion promotes UV-mediated transformation of human melanocytes, and its inactivation may contribute to oncogenic transformation [6].
In conclusion, FBH1 is vital for maintaining genome integrity through its functions in replication stress response, homologous recombination regulation, and checkpoint control. The study of FBH1-deficient models has revealed its significance in cancer-related processes, such as oncogenic transformation and response to WEE1 inhibition, providing insights into potential cancer therapeutic strategies.
References：
1. Jennings, Lucy, Walters, Heather Andrews, Mason, Jennifer M. 2023. FBH1 deficiency sensitizes cells to WEE1 inhibition by promoting mitotic catastrophe. In bioRxiv : the preprint server for biology, , . doi:10.1101/2023.05.15.540841. https://pubmed.ncbi.nlm.nih.gov/37292855/
2. Liu, Guangxue, Li, Jimin, He, Boxue, Zhang, Xinghua, Chen, Xuefeng. 2023. Bre1/RNF20 promotes Rad51-mediated strand exchange and antagonizes the Srs2/FBH1 helicases. In Nature communications, 14, 3024. doi:10.1038/s41467-023-38617-z. https://pubmed.ncbi.nlm.nih.gov/37230987/
3. Liu, Jiuyang, Chaves-Arquero, Belén, Wei, Pengcheng, Blanco, Francisco J, Kutateladze, Tatiana G. 2023. Molecular insight into the PCNA-binding mode of FBH1. In Structure (London, England : 1993), 31, 511-517.e3. doi:10.1016/j.str.2023.03.004. https://pubmed.ncbi.nlm.nih.gov/36990095/
4. Hawks, Alexandra L, Bergmann, Amy, McCraw, Tyler J, Mason, Jennifer M. 2023. UBC13-mediated template switching promotes replication stress resistance in FBH1-deficient cells. In bioRxiv : the preprint server for biology, , . doi:10.1101/2023.09.04.556280. https://pubmed.ncbi.nlm.nih.gov/37732269/
5. Jennings, Lucy, Walters, Heather Andrews, McCraw, Tyler J, Turner, Joshua L, Mason, Jennifer M. 2023. FBH1 deficiency sensitizes cells to WEE1 inhibition by promoting mitotic catastrophe. In DNA repair, 133, 103611. doi:10.1016/j.dnarep.2023.103611. https://pubmed.ncbi.nlm.nih.gov/38103522/
6. Jeong, Yeon-Tae, Cermak, Lukas, Guijarro, Maria V, Hernando, Eva, Pagano, Michele. 2013. FBH1 protects melanocytes from transformation and is deregulated in melanomas. In Cell cycle (Georgetown, Tex.), 12, 1128-32. doi:10.4161/cc.24165. https://pubmed.ncbi.nlm.nih.gov/23466708/
7. Fugger, Kasper, Mistrik, Martin, Neelsen, Kai J, Hickson, Ian D, Sørensen, Claus Storgaard. 2015. FBH1 Catalyzes Regression of Stalled Replication Forks. In Cell reports, 10, 1749-1757. doi:10.1016/j.celrep.2015.02.028. https://pubmed.ncbi.nlm.nih.gov/25772361/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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