C57BL/6JCya-Adam10em1/Cya
Common Name:
Adam10-KO
Product ID:
S-KO-19201
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Adam10-KO
Strain ID
KOCMP-11487-Adam10-B6J-VA
Gene Name
Product ID
S-KO-19201
Gene Alias
1700031C13Rik; MADM; kuz; kuzbanian
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
9
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Adam10em1/Cya mice (Catalog S-KO-19201) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000067880
NCBI RefSeq
NM_007399
Target Region
Exon 3
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
ADAM10, a member of the A Disintegrin And Metalloproteinase (ADAM) superfamily, is a membrane-bound protein with its catalytic domain on the cell surface. It functions as a sheddase, cleaving over 100 anchored cell-surface proteins, and is involved in crucial pathways such as Notch signaling [2,3,6]. ADAM10 is expressed in most tissues and plays important roles in mammalian development, physiology, and is also the primary α-secretase in the non-amyloidogenic cleavage of APP [3,5].
In the context of diseases, ADAM10 has been studied in various models. In hair follicles, ablation of the ADAM10-Notch signaling axis in mice led to skin dysbiosis, innate lymphoid cell-mediated hair follicle destruction, and irreversible alopecia, indicating its role in maintaining skin microbiota and protecting hair follicles from inflammation [6]. In renal diseases, ADAM10 is closely associated with the progress of glomerular diseases, acute kidney injury, and renal fibrosis, and may be a potential therapeutic target [1]. In cancer, ADAM10 cleavage of Trop-2 acts as an activator switch for cancer growth and metastasis, and it also modulates the efficacy of T-cell-mediated therapy in solid tumors [7,8]. In sepsis, ADAM10 acts as a "molecular scissor" regulating the inflammatory response by cleaving various membrane-protein substrates, though its substrate-specific modulation in sepsis is yet to be fully elucidated [4]. In Alzheimer's disease, ADAM10 levels are altered in platelets, plasma, serum, and CSF, potentially making it a complementary biomarker, but more studies are needed to establish cut-off values [9].
In conclusion, ADAM10 is a multifunctional protein involved in diverse biological processes. Studies using gene-knockout or conditional-knockout mouse models have revealed its significance in multiple disease areas, including skin disorders, renal diseases, cancer, sepsis, and Alzheimer's disease. These findings highlight the potential of ADAM10 as a therapeutic target and biomarker in these diseases.
References:
1. Wang, Jie-Na, Cao, Xiao-Jing. . Targeting ADAM10 in Renal Diseases. In Current molecular medicine, 23, 1037-1045. doi:10.2174/1566524023666221020142504. https://pubmed.ncbi.nlm.nih.gov/36278450/
2. Shahid, Shifa, Ikeda, Atsushi, Layana, Michelle C, Bartlett, John D. 2022. ADAM10: Possible functions in enamel development. In Frontiers in physiology, 13, 1032383. doi:10.3389/fphys.2022.1032383. https://pubmed.ncbi.nlm.nih.gov/36505044/
3. Lipper, Colin H, Egan, Emily D, Gabriel, Khal-Hentz, Blacklow, Stephen C. 2023. Structural basis for membrane-proximal proteolysis of substrates by ADAM10. In Cell, 186, 3632-3641.e10. doi:10.1016/j.cell.2023.06.026. https://pubmed.ncbi.nlm.nih.gov/37516108/
4. Liao, Shuanglin, Lin, Yao, Liu, Lizhen, He, Junbing, Shao, Yiming. 2022. ADAM10-a "multitasker" in sepsis: focus on its posttranslational target. In Inflammation research : official journal of the European Histamine Research Society ... [et al.], 72, 395-423. doi:10.1007/s00011-022-01673-0. https://pubmed.ncbi.nlm.nih.gov/36565333/
5. Dorta, Sabrina, Alexandre-Silva, Vanessa, Popolin, Cecilia Patricia, Endres, Kristina, Cominetti, Marcia Regina. 2024. ADAM10 isoforms: Optimizing usage of antibodies based on protein regulation, structural features, biological activity and clinical relevance to Alzheimer's disease. In Ageing research reviews, 101, 102464. doi:10.1016/j.arr.2024.102464. https://pubmed.ncbi.nlm.nih.gov/39173916/
6. Sakamoto, Keiko, Jin, Seon-Pil, Goel, Shubham, Kong, Heidi H, Nagao, Keisuke. 2021. Disruption of the endopeptidase ADAM10-Notch signaling axis leads to skin dysbiosis and innate lymphoid cell-mediated hair follicle destruction. In Immunity, 54, 2321-2337.e10. doi:10.1016/j.immuni.2021.09.001. https://pubmed.ncbi.nlm.nih.gov/34582748/
7. Trerotola, Marco, Guerra, Emanuela, Ali, Zeeshan, Lattanzio, Rossano, Alberti, Saverio. 2021. Trop-2 cleavage by ADAM10 is an activator switch for cancer growth and metastasis. In Neoplasia (New York, N.Y.), 23, 415-428. doi:10.1016/j.neo.2021.03.006. https://pubmed.ncbi.nlm.nih.gov/33839455/
8. Abdalla, Ahmed Me, Miao, Yu, Ming, Ning, Ouyang, Chenxi. 2024. ADAM10 modulates the efficacy of T-cell-mediated therapy in solid tumors. In Immunology and cell biology, 102, 907-923. doi:10.1111/imcb.12826. https://pubmed.ncbi.nlm.nih.gov/39417304/
9. Oliveira Monteiro E Pereira de Almeida, M P, Valle Pedroso, R, Mantellatto Grigoli, M, Manzine, P R, Cominetti, M R. 2023. ADAM10 as a biomarker for Alzheimer's disease: A systematic review. In Revue neurologique, 180, 1-11. doi:10.1016/j.neurol.2023.04.002. https://pubmed.ncbi.nlm.nih.gov/37460331/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen