C57BL/6JCya-Itgb5em1/Cya
Common Name:
Itgb5-KO
Product ID:
S-KO-19287
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Itgb5-KO
Strain ID
KOCMP-16419-Itgb5-B6J-VC
Gene Name
Product ID
S-KO-19287
Gene Alias
ESTM23; [b]-5; [b]5; [b]5A; [b]5B; beta-5; beta5
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
16
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Itgb5em1/Cya mice (Catalog S-KO-19287) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000069345
NCBI RefSeq
NM_010580
Target Region
Exon 5
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Itgb5, encoding integrin β5 subunit, is crucial in cell adhesion and binding. It assorts with the αv subunit to form integrin αvβ5, and is involved in multiple signaling pathways like integrin signaling, which is essential for various biological processes such as cell-matrix interactions, cell migration, and tissue development [3].
In multiple cancers, Itgb5 shows oncogenic roles. In gastric cancer, knockdown of Itgb5 impairs cell metastasis both in vitro and in vivo, as Itgb5 promotes TGFBR2 endosomal recycling, facilitating epithelial-mesenchymal transition mediated by TGFβ signaling [1]. In pancreatic adenocarcinoma, knocking down Itgb5 increases cell sensitivity to radiation by influencing DNA damage repair and the MEK/ERK signaling pathway [2]. In hepatocellular carcinoma, Itgb5 confers sorafenib resistance through a positive feedback loop involving EPS15, EGFR, and CSNK1A1 [4]. In colorectal cancer, silencing Itgb5 inhibits cell proliferation, invasion, and migration by blocking the Wnt/β-catenin signaling pathway and epithelial-mesenchymal transition [5]. In addition, in piglets, disruption of Itgb5 in IPEC-J2 cell lines using Nuclease technology significantly reduces the adhesion of enterotoxigenic Escherichia coli F4ac to porcine intestinal epithelial cells, suggesting its role in ETEC F4ac-induced diarrhea [3].
In conclusion, Itgb5 is mainly involved in cell adhesion-related functions and plays important roles in multiple disease conditions, especially in promoting cancer metastasis and drug resistance. Studies using gene knockout or knockdown models in various organisms and cell lines have been crucial in revealing these functions, providing potential therapeutic targets for cancers and insights into other diseases like ETEC-induced diarrhea in piglets.
References:
1. Lei, Tianxiang, Lin, Yingying, Lai, Xiaofan, Feng, Wei, Song, Wu. 2024. ITGB5 facilitates gastric cancer metastasis by promoting TGFBR2 endosomal recycling. In Cancer letters, 592, 216953. doi:10.1016/j.canlet.2024.216953. https://pubmed.ncbi.nlm.nih.gov/38729557/
2. Wen, Xin, Chen, Si, Chen, Xueting, Ding, Xin, Zhang, Longzhen. 2022. ITGB5 promotes innate radiation resistance in pancreatic adenocarcinoma by promoting DNA damage repair and the MEK/ERK signaling pathway. In Frontiers in oncology, 12, 887068. doi:10.3389/fonc.2022.887068. https://pubmed.ncbi.nlm.nih.gov/36249018/
3. Wang, Wenwen, Liu, Yang, Tang, Hui, Yu, Ying, Zhang, Qin. 2019. ITGB5 Plays a Key Role in Escherichia coli F4ac-Induced Diarrhea in Piglets. In Frontiers in immunology, 10, 2834. doi:10.3389/fimmu.2019.02834. https://pubmed.ncbi.nlm.nih.gov/31921118/
4. Gu, Li, Jin, Xin, Liang, Huaiyuan, Yang, Chong, Zhang, Yu. 2023. Upregulation of CSNK1A1 induced by ITGB5 confers to hepatocellular carcinoma resistance to sorafenib in vivo by disrupting the EPS15/EGFR complex. In Pharmacological research, 192, 106789. doi:10.1016/j.phrs.2023.106789. https://pubmed.ncbi.nlm.nih.gov/37149115/
5. Chen, Zhihua, Fang, Yuan, Zhong, Shuwu, Yang, Xiaoyu, Chen, Shaoqin. 2025. ITGB5 is a prognostic factor in colorectal cancer and promotes cancer progression and metastasis through the Wnt signaling pathway. In Scientific reports, 15, 9225. doi:10.1038/s41598-025-93081-7. https://pubmed.ncbi.nlm.nih.gov/40097546/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen