C57BL/6JCya-Taf11em1/Cya
Common Name:
Taf11-KO
Product ID:
S-KO-19395
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Taf11-KO
Strain ID
KOCMP-68776-Taf11-B6J-VC
Gene Name
Product ID
S-KO-19395
Gene Alias
1110038O14Rik; PRO214; TAF2I; TAFII28
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
17
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Taf11em1/Cya mice (Catalog S-KO-19395) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000114848
NCBI RefSeq
NM_026836
Target Region
Exon 3
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
Taf11, short for TATA-binding protein-associated factor 11, is involved in multiple biological processes. It is known to be an important factor in the assembly of the RISC loading complex (RLC) in Drosophila, which enhances RNAi efficiency. Taf11 also participates in the regulation of gene expression as part of the general transcription factor TFIID, and is associated with pathways related to cell adhesion, neural crest cell migration, and may be involved in processes relevant to rheumatoid arthritis-associated interstitial lung disease [1,2,3,4].
In Drosophila, genetic screening identified Taf11 as a missing factor of RLC. Taf11(-/-) ovary extract showed defective RLC assembly, and in vitro reconstitution demonstrated that Taf11 tetramer facilitates Dcr-2-R2D2 tetramerization to enhance siRNA binding and RISC loading activities [1]. In zebrafish, overexpression of Taf11 contributed to disturbed migration of cranial neural crest cells and abnormal craniofacial development, suggesting its role in these processes [2].
In conclusion, Taf11 is essential for RLC assembly to enhance RNAi efficiency and plays a role in regulating cell migration during craniofacial development. The use of Drosophila and zebrafish as research models has provided insights into these functions, which may have implications for understanding related biological processes and potentially associated diseases.
References:
1. Liang, Chunyang, Wang, Yibing, Murota, Yukiko, Siomi, Mikiko C, Liu, Qinghua. 2015. TAF11 Assembles the RISC Loading Complex to Enhance RNAi Efficiency. In Molecular cell, 59, 807-18. doi:10.1016/j.molcel.2015.07.006. https://pubmed.ncbi.nlm.nih.gov/26257286/
2. Li, Dandan, Tian, Yu, Vona, Barbara, Wang, Lin, Pan, Yongchu. . A TAF11 variant contributes to non-syndromic cleft lip only through modulating neural crest cell migration. In Human molecular genetics, 34, 392-401. doi:10.1093/hmg/ddae188. https://pubmed.ncbi.nlm.nih.gov/39727181/
3. Gupta, Kapil, Watson, Aleksandra A, Baptista, Tiago, Tora, Làszlò, Berger, Imre. 2017. Architecture of TAF11/TAF13/TBP complex suggests novel regulation properties of general transcription factor TFIID. In eLife, 6, . doi:10.7554/eLife.30395. https://pubmed.ncbi.nlm.nih.gov/29111974/
4. Liu, Muqiu, Jiang, Zhihao, Liu, Min, Du, Qun, Dong, Yan. 2024. SLAMF1 as a novel molecule mediating the causal association between rheumatoid arthritis and interstitial lung disease: A Mendelian randomization study combined with transcriptomics and in vivo validation. In International immunopharmacology, 142, 113082. doi:10.1016/j.intimp.2024.113082. https://pubmed.ncbi.nlm.nih.gov/39260308/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen