C57BL/6JCya-Mapk12em1/Cya
Common Name:
Mapk12-KO
Product ID:
S-KO-19494
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Mapk12-KO
Strain ID
KOCMP-29857-Mapk12-B6J-VB
Gene Name
Product ID
S-KO-19494
Gene Alias
Erk6; P38gamma; Prkm12; Sapk3
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
15
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mapk12em1/Cya mice (Catalog S-KO-19494) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000088827
NCBI RefSeq
NM_013871
Target Region
Exon 3
Size of Effective Region
~1.7 kb
Detailed Document
Overview of Gene Research
Mapk12, also known as P38γ, is a member of the p38 MAPK family. It is involved in various cellular processes, such as cell proliferation, apoptosis, stress responses, and cancer development, and plays a crucial role in the regulation of the innate immune response [2,4,5]. Genetic models, like gene knockout mouse models, are valuable for studying its functions.
In osteoarthritis (OA), downregulation of Mapk12 was observed in H2O2-or IL-1β-stimulated cells and OA-related tissues. Over-expression of Mapk12 promoted cell proliferation and cartilage anabolism, inhibited cell senescence and cartilage catabolism, and relieved joint pain in relevant cell models and DMM mice, indicating its involvement in OA development through modulating chondrocyte senescence [1].
In cancer, pan-cancer analysis showed Mapk12 was overexpressed in multiple cancer types, and its upregulated mRNA expression was related to a worse prognosis. Knockdown of Mapk12 inhibited thyroid carcinoma cell proliferation, and its gene enrichment was related to cell proliferation and the tumor immune microenvironment. It was also closely related to immune checkpoint, microsatellite instability, and tumor mutational burden, affecting tumor sensitivity to immunotherapy [2].
In diffuse large B-cell lymphoma (DLBCL), high expression of Mapk12 was significantly correlated with shorter progression-free survival and overall survival, and was involved in the regulation of type II interferon production and lymphocyte proliferation [6].
In osteosarcoma, miR-187 inhibited tumor growth and invasion by directly targeting Mapk12 [7].
In ovine ovarian granulosa cells, high prolactin concentration promoted apoptosis and inhibited steroid hormone secretion by upregulating Mapk12 [3].
In conclusion, Mapk12 is a key regulator in multiple biological processes and diseases. Studies using gene-based models have revealed its role in OA, various cancers, and ovarian granulosa cell apoptosis. Understanding Mapk12 functions can provide potential therapeutic targets for these diseases.
References：
1. Geng, Nana, Xian, Menglin, Deng, Lin, Bai, Zhixun, Guo, Fengjin. 2024. Targeting the senescence-related genes MAPK12 and FOS to alleviate osteoarthritis. In Journal of orthopaedic translation, 47, 50-62. doi:10.1016/j.jot.2024.06.008. https://pubmed.ncbi.nlm.nih.gov/39007035/
2. Wang, Jinju, Song, Zhe, Ren, Li, Gu, Yi, Feng, Chao. 2022. Pan-cancer analysis supports MAPK12 as a potential prognostic and immunotherapeutic target in multiple tumor types, including in THCA. In Oncology letters, 24, 445. doi:10.3892/ol.2022.13565. https://pubmed.ncbi.nlm.nih.gov/36420075/
3. Yang, Ruochen, Duan, Chunhui, Zhang, Shuo, Liu, Yueqin, Zhang, Yingjie. 2023. Prolactin Regulates Ovine Ovarian Granulosa Cell Apoptosis by Affecting the Expression of MAPK12 Gene. In International journal of molecular sciences, 24, . doi:10.3390/ijms241210269. https://pubmed.ncbi.nlm.nih.gov/37373417/
4. Escós, Alejandra, Diaz-Mora, Ester, Pattison, Michael, Ley, Steven C, Cuenda, Ana. 2023. p38γ and p38δ modulate innate immune response by regulating MEF2D activation. In eLife, 12, . doi:10.7554/eLife.86200. https://pubmed.ncbi.nlm.nih.gov/37458356/
5. Phan, Thuy, Zhang, Xu Hannah, Rosen, Steven, Melstrom, Laleh G. 2023. P38 kinase in gastrointestinal cancers. In Cancer gene therapy, 30, 1181-1189. doi:10.1038/s41417-023-00622-1. https://pubmed.ncbi.nlm.nih.gov/37248432/
6. Liu, Yue, Zhang, Han, Zhao, Shu, Zhang, Yue. 2024. A retrospective analysis of the clinicopathological features and prognostic value of MAPK12 protein expression in diffuse large B-cell lymphoma. In Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico, 26, 2966-2978. doi:10.1007/s12094-024-03515-3. https://pubmed.ncbi.nlm.nih.gov/38773060/
7. Cui, Chengliang, Shi, Xiaoyu. 2017. miR-187 inhibits tumor growth and invasion by directly targeting MAPK12 in osteosarcoma. In Experimental and therapeutic medicine, 14, 1045-1050. doi:10.3892/etm.2017.4624. https://pubmed.ncbi.nlm.nih.gov/28810556/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen