C57BL/6JCya-Tktl1em1/Cya
Common Name:
Tktl1-KO
Product ID:
S-KO-19495
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Tktl1-KO
Strain ID
KOCMP-83553-Tktl1-B6J-VB
Gene Name
Product ID
S-KO-19495
Gene Alias
-
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
X
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tktl1em1/Cya mice (Catalog S-KO-19495) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000010127
NCBI RefSeq
NM_031379
Target Region
Exon 4~5
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
Tktl1, or transketolase-like 1, is a crucial regulatory enzyme in the pentose phosphate pathway (PPP), playing a significant role in energy synthesis [4]. It is also associated with anaerobic glucose metabolism (Warburg effect) [3]. Three transketolase genes, including Tktl1, have been identified in the human genome, and homology modelling suggests Tktl1 is a functional transketolase, potentially a novel therapeutic target for diabetes and cancer [7].
In modern humans, Tktl1 differs from Neanderthal Tktl1 by a lysine-to-arginine amino acid substitution. Overexpression in developing mouse neocortex and knockout in fetal human neocortical tissue, along with experiments in genome-edited cerebral organoids, show that the modern human variant of Tktl1 increases the abundance of basal radial glia (bRG), which generate more neocortical neurons, suggesting differences in neocortical neurogenesis between modern humans and Neanderthals [1]. In THP-1 AML cells, knockdown of Tktl1 impairs hypoxia-induced overexpression of glucose-6-phosphate dehydrogenase (G6PD) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH), affecting the cell's redox capacity and its ability to adapt to hypoxia [2]. In paclitaxel-resistant human ovarian cancer cells, inhibition of Tktl1 re-sensitizes the cells to paclitaxel, reducing cellular proliferation and increasing apoptosis [5]. In cervical cancer cells, interference of Tktl1 expression inhibits cell proliferation, invasion, migration, and glycolysis [6].
In summary, Tktl1 is essential in the pentose phosphate pathway and glucose metabolism. Studies using gene knockout and knockdown models have revealed its role in various biological processes such as neurogenesis, cell response to hypoxia, and cancer cell behavior, highlighting its significance in understanding human evolution and treating cancer.
References:
1. Pinson, Anneline, Xing, Lei, Namba, Takashi, Pääbo, Svante, Huttner, Wieland B. 2022. Human TKTL1 implies greater neurogenesis in frontal neocortex of modern humans than Neanderthals. In Science (New York, N.Y.), 377, eabl6422. doi:10.1126/science.abl6422. https://pubmed.ncbi.nlm.nih.gov/36074851/
2. Baptista, Inês, Karakitsou, Effrosyni, Cazier, Jean-Baptiste, Marin, Silvia, Cascante, Marta. 2022. TKTL1 Knockdown Impairs Hypoxia-Induced Glucose-6-phosphate Dehydrogenase and Glyceraldehyde-3-phosphate Dehydrogenase Overexpression. In International journal of molecular sciences, 23, . doi:10.3390/ijms23073574. https://pubmed.ncbi.nlm.nih.gov/35408935/
3. Coy, Johannes F. 2017. EDIM-TKTL1/Apo10 Blood Test: An Innate Immune System Based Liquid Biopsy for the Early Detection, Characterization and Targeted Treatment of Cancer. In International journal of molecular sciences, 18, . doi:10.3390/ijms18040878. https://pubmed.ncbi.nlm.nih.gov/28425973/
4. Yuan, Xiaole, Kang, Huaiyan, Liu, Bo, Chen, Shenghui, Du, Xiaomin. . Molecular Characteristics and Expression of TKTL1 in Germ Cells: Implications for Nontumour Cell Research. In Reproduction in domestic animals = Zuchthygiene, 59, e14723. doi:10.1111/rda.14723. https://pubmed.ncbi.nlm.nih.gov/39311634/
5. Zheng, Xing, Li, Hongxia. 2018. TKTL1 modulates the response of paclitaxel-resistant human ovarian cancer cells to paclitaxel. In Biochemical and biophysical research communications, 503, 572-579. doi:10.1016/j.bbrc.2018.06.011. https://pubmed.ncbi.nlm.nih.gov/29885837/
6. Zhu, Yingping, Qiu, Yu, Zhang, Xueqin. 2021. TKTL1 participated in malignant progression of cervical cancer cells via regulating AKT signal mediated PFKFB3 and thus regulating glycolysis. In Cancer cell international, 21, 678. doi:10.1186/s12935-021-02383-z. https://pubmed.ncbi.nlm.nih.gov/34922556/
7. Deshpande, Gaurang P, Patterton, Hugh-George, Faadiel Essop, M. 2019. The human transketolase-like proteins TKTL1 and TKTL2 are bona fide transketolases. In BMC structural biology, 19, 2. doi:10.1186/s12900-018-0099-y. https://pubmed.ncbi.nlm.nih.gov/30646877/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen