C57BL/6JCya-Gnpda1em1/Cya
Common Name:
Gnpda1-KO
Product ID:
S-KO-19587
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Contact for Pricing
Basic Information
Strain Name
Gnpda1-KO
Strain ID
KOCMP-26384-Gnpda1-B6J-VA
Gene Name
Product ID
S-KO-19587
Gene Alias
GNPDA; Gnp1; Gnpi; oscillin
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
18
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gnpda1em1/Cya mice (Catalog S-KO-19587) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000063814
NCBI RefSeq
NM_011937
Target Region
Exon 4~5
Size of Effective Region
~3.0 kb
Detailed Document
Overview of Gene Research
Gnpda1, glucosamine-6-phosphate deaminase 1, is an enzyme involved in the hexosamine biosynthetic pathway (HBP). It can convert glucosamine-6-phosphate (GlcN6P) back into fructose-6-phosphate (Fru6P) and ammonium, thus affecting protein N-glycosylation and O-linked-N-acetylglucosamine modification (O-GlcNAcylation), which are crucial for protein function and cellular responses to signals. The HBP and Gnpda1 are associated with metabolic diseases and cancer [3].
In hepatocellular carcinoma (HCC), Gnpda1 is highly expressed. High Gnpda1 expression is significantly associated with advanced tumor stage, TNM stage or grade, and is related to poor prognosis in HCC patients. Knockdown of Gnpda1 in SMMC-7721 and Huh7 cell lines inhibits the proliferation, migration, and invasion of HCC cells and promotes apoptosis, suggesting it may be a novel prognostic biomarker and therapeutic target for HCC [1].
In addition, Gnpda1 was identified as one of the up-regulated genes in the phlegm-dampness constitution, and gene functional analyses indicated that people with this constitution are susceptible to hyperlipemia and diabetes [2].
In colorectal cancer cells treated with caffeic acid phenethyl ester (CAPE), Gnpda1 was up-regulated, and these differentiated proteins may be potential molecular targets involved in the anti-cancer effect of CAPE [4].
An integrative analysis revealed Gnpda1 as a potential gene related to biopsychosocial factors among Taiwanese women with a family history of breast cancer. Gnpda1 hypomethylation was associated with poor overall survival in breast cancer, and it was also associated with the BRCA1, BRCA2, and pro-oncogenic actions of the androgen receptor in breast cancer [5].
A study on glycolysis-related gene pairs signature found that the IDUA_GNPDA1 pair was a significantly progressive factor for HCC patients, and a five-gene expression signature including Gnpda1 had a satisfactory prognostic value for overall survival of gastrointestinal cancer patients in the Asian population [6,7].
In gemcitabine-resistant pancreatic cancer cells, Gnpda1 was one of the mRNAs targeted by specific miRNAs, and gemcitabine resistance may alter the fraction of memory CD4+ T cells via the miRNA-Gnpda1 network [8].
In conclusion, Gnpda1 is an important enzyme in the hexosamine biosynthetic pathway. Its abnormal expression is closely related to the occurrence and development of various cancers such as HCC, breast cancer, colorectal cancer, and pancreatic cancer, as well as metabolic diseases like hyperlipemia and diabetes. Studies on Gnpda1 contribute to understanding the mechanisms of these diseases and may provide new directions for diagnosis, prognosis, and treatment.
References:
1. Li, Dezhi, Cheng, Xianyi, Zheng, Wei, Chen, Junhui. 2020. Glucosamine-6-Phosphate Isomerase 1 Promotes Tumor Progression and Indicates Poor Prognosis in Hepatocellular Carcinoma. In Cancer management and research, 12, 4923-4935. doi:10.2147/CMAR.S250094. https://pubmed.ncbi.nlm.nih.gov/32606980/
2. Wang, Ji, Wang, Qi, Li, Lingru, Zhang, Yan, Han, Yuanyuan. . Phlegm-dampness constitution: genomics, susceptibility, adjustment and treatment with traditional Chinese medicine. In The American journal of Chinese medicine, 41, 253-62. doi:10.1142/S0192415X13500183. https://pubmed.ncbi.nlm.nih.gov/23548117/
3. Lara-Lemus, Roberto, Castillejos-López, Manuel, Aquino-Gálvez, Arnoldo. 2024. The Possible Roles of Glucosamine-6-Phosphate Deaminases in Ammonium Metabolism in Cancer. In International journal of molecular sciences, 25, . doi:10.3390/ijms252212054. https://pubmed.ncbi.nlm.nih.gov/39596123/
4. He, Yu-Jun, Li, Wan-Ling, Liu, Bao-Hua, Mou, Zhi-Rong, Wu, Yu-Zhang. . Identification of differential proteins in colorectal cancer cells treated with caffeic acid phenethyl ester. In World journal of gastroenterology, 20, 11840-9. doi:10.3748/wjg.v20.i33.11840. https://pubmed.ncbi.nlm.nih.gov/25206290/
5. Khairi, Sabiah, Wang, Chih-Yang, Anuraga, Gangga, Shen, Chen-Yang, Chung, Min-Huey. 2025. Integrative Analysis of DNA Methylation and microRNA Reveals GNPDA1 and SLC25A16 Related to Biopsychosocial Factors Among Taiwanese Women with a Family History of Breast Cancer. In Journal of personalized medicine, 15, . doi:10.3390/jpm15040134. https://pubmed.ncbi.nlm.nih.gov/40278313/
6. Zhou, Weige, Zhang, Shijing, Cai, Zheyou, Hou, Zheng-Kun, Chen, Xin-Lin. 2020. A glycolysis-related gene pairs signature predicts prognosis in patients with hepatocellular carcinoma. In PeerJ, 8, e9944. doi:10.7717/peerj.9944. https://pubmed.ncbi.nlm.nih.gov/33062428/
7. Xia, Rong, Tang, Hua, Shen, Jiemiao, Yang, Jinyou, Wang, Chao. 2021. Prognostic value of a novel glycolysis-related gene expression signature for gastrointestinal cancer in the Asian population. In Cancer cell international, 21, 154. doi:10.1186/s12935-021-01857-4. https://pubmed.ncbi.nlm.nih.gov/33663535/
8. Gu, Jianyou, Zhang, Junfeng, Huang, Wenjie, Fan, Yingfang, Wang, Huaizhi. . Activating miRNA-mRNA network in gemcitabine-resistant pancreatic cancer cell associates with alteration of memory CD4+ T cells. In Annals of translational medicine, 8, 279. doi:10.21037/atm.2020.03.53. https://pubmed.ncbi.nlm.nih.gov/32355723/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen