C57BL/6JCya-Gspt1em1/Cya
Common Name:
Gspt1-KO
Product ID:
S-KO-19830
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Gspt1-KO
Strain ID
KOCMP-14852-Gspt1-B6J-VA
Gene Name
Product ID
S-KO-19830
Gene Alias
G1st; Gst-1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
16
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gspt1em1/Cya mice (Catalog S-KO-19830) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000080030
NCBI RefSeq
NM_146066
Target Region
Exon 4~5
Size of Effective Region
~2.5 kb
Detailed Document
Overview of Gene Research
Gspt1, also known as G1 to S phase transition 1, is a key translation termination factor [2,4]. It interacts with eRF1 to facilitate the translation termination process, which is crucial for normal protein synthesis and cellular function [4].
Studies have shown that Gspt1 is significantly overexpressed in various cancer tissues and cells, including acute myeloid leukemia, MYC-driven lung cancer, liver cancer, and glioblastoma [2,4,5,6]. Drugs that degrade Gspt1 via the CRL4CRBN ubiquitin ligase are a new class of cancer therapy in active clinical development [3]. For example, CC-885, a cereblon modulator, can recruit Gspt1 to the CRL4(CRBN) ubiquitin ligase, leading to Gspt1's ubiquitination and degradation, which mediates its anti-tumor activity [1]. In acute myeloid leukemia, Gspt1 degradation leads to impaired translation termination, activation of the integrated stress response pathway, and TP53-independent cell death, while sparing normal hematopoietic stem cells [3]. In glioblastoma, depletion of Gspt1 can enhance apoptosis and prolong the survival period of nude mice with transplanted brain tumors [6].
In conclusion, Gspt1 is essential for normal translation termination. Its overexpression in multiple cancers makes it a promising anti-tumor target. Studies using Gspt1-degrading drugs in mouse models have revealed its role in cancer cell death and the sparing of normal hematopoietic stem cells, providing a mechanistic basis for using Gspt1 degraders in cancer treatment, especially for TP53-mutant acute myeloid leukemia [3].
References:
1. Matyskiela, Mary E, Lu, Gang, Ito, Takumi, Lopez-Girona, Antonia, Chamberlain, Philip P. 2016. A novel cereblon modulator recruits GSPT1 to the CRL4(CRBN) ubiquitin ligase. In Nature, 535, 252-7. doi:10.1038/nature18611. https://pubmed.ncbi.nlm.nih.gov/27338790/
2. Zhang, Dandan, Lin, Pei, Lin, Jun. 2023. Molecular glues targeting GSPT1 in cancers: A potent therapy. In Bioorganic chemistry, 143, 107000. doi:10.1016/j.bioorg.2023.107000. https://pubmed.ncbi.nlm.nih.gov/38029571/
3. Sellar, Rob S, Sperling, Adam S, Słabicki, Mikołaj, Chen, Chun-Wei, Ebert, Benjamin L. . Degradation of GSPT1 causes TP53-independent cell death in leukemia while sparing normal hematopoietic stem cells. In The Journal of clinical investigation, 132, . doi:10.1172/JCI153514. https://pubmed.ncbi.nlm.nih.gov/35763353/
4. Chang, Xiujin, Qu, Fangui, Li, Chunxiao, Li, Zhiyu, Xu, Xi. 2024. Development and therapeutic potential of GSPT1 molecular glue degraders: A medicinal chemistry perspective. In Medicinal research reviews, 44, 1727-1767. doi:10.1002/med.22024. https://pubmed.ncbi.nlm.nih.gov/38314926/
5. Xi, Yi-Qing, Gao, Jing-Bo, Li, Xuan-Fei, Feng, Mao-Hui, Zhang, Jing-Wei. 2022. GSPT1 Functions as a Tumor Promoter in Human Liver Cancer. In Current medical science, 43, 104-114. doi:10.1007/s11596-022-2665-6. https://pubmed.ncbi.nlm.nih.gov/36459303/
6. Sasayama, Takashi, Hamada, Takeshi, Tanaka, Kazuhiro, Yamanishi, Shunsuke, Ueyama, Takehiko. 2024. Potential of GSPT1 as a novel target for glioblastoma therapy. In Cell death & disease, 15, 572. doi:10.1038/s41419-024-06967-1. https://pubmed.ncbi.nlm.nih.gov/39117611/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen