C57BL/6JCya-Coq3em1/Cya
Common Name:
Coq3-KO
Product ID:
S-KO-19897
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Coq3-KO
Strain ID
KOCMP-230027-Coq3-B6J-VB
Gene Name
Product ID
S-KO-19897
Gene Alias
4732433J24
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Coq3em1/Cya mice (Catalog S-KO-19897) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000029909
NCBI RefSeq
NM_172687
Target Region
Exon 4~5
Size of Effective Region
~2.0 kb
Detailed Document
Overview of Gene Research
Coq3, encoding 3,4-dihydroxy-5-polyprenylbenzoate methyltransferase, is crucial for coenzyme Q (Q) biosynthesis [2,3,5,7]. Q is a redox-active lipid essential for aerobic respiration in eukaryotes, with Coq3 catalyzing two O-methylation steps in the Q biosynthetic pathway [3,4,6].
In yeast, Coq3 and Coq4 form a high-molecular-mass complex for Q biosynthesis [1]. Coq3, along with other Coq polypeptides like Coq1, Coq5, and Coq6, is part of a larger complex involved in Q production [1]. The Arabidopsis homologue AtCOQ3 also functions in mitochondrial ubiquinone synthesis [7]. Escherichia coli UbiG, an orthologue of Coq3, can complement yeast coq3 mutants and catalyze both O-methylation steps [4]. The activity and expression of Coq3 O-methyltransferase depend on other COQ gene products, suggesting a multi-subunit complex in Q biosynthesis [8].
In conclusion, Coq3 is essential for coenzyme Q biosynthesis, a process conserved across species from yeast to plants and mammals. Understanding Coq3 function through model-based research, such as in yeast mutants, helps in revealing its role in maintaining aerobic respiration and may have implications for understanding diseases related to Q deficiencies, like the symptoms seen in mouse mutants similar to human Q-deficiency conditions [2].
References：
1. Marbois, Beth, Gin, Peter, Faull, Kym F, Shepherd, Jennifer N, Clarke, Catherine F. 2005. Coq3 and Coq4 define a polypeptide complex in yeast mitochondria for the biosynthesis of coenzyme Q. In The Journal of biological chemistry, 280, 20231-8. doi:. https://pubmed.ncbi.nlm.nih.gov/15792955/
2. Marbois, B N, Xia, Y R, Lusis, A J, Clarke, C F. . Ubiquinone biosynthesis in eukaryotic cells: tissue distribution of mRNA encoding 3,4-dihydroxy-5-polyprenylbenzoate methyltransferase in the rat and mapping of the COQ3 gene to mouse chromosome 4. In Archives of biochemistry and biophysics, 313, 83-8. doi:. https://pubmed.ncbi.nlm.nih.gov/8053692/
3. Jonassen, T, Clarke, C F. . Isolation and functional expression of human COQ3, a gene encoding a methyltransferase required for ubiquinone biosynthesis. In The Journal of biological chemistry, 275, 12381-7. doi:. https://pubmed.ncbi.nlm.nih.gov/10777520/
4. Hsu, A Y, Poon, W W, Shepherd, J A, Myles, D C, Clarke, C F. . Complementation of coq3 mutant yeast by mitochondrial targeting of the Escherichia coli UbiG polypeptide: evidence that UbiG catalyzes both O-methylation steps in ubiquinone biosynthesis. In Biochemistry, 35, 9797-806. doi:. https://pubmed.ncbi.nlm.nih.gov/8703953/
5. Clarke, C F, Williams, W, Teruya, J H. . Ubiquinone biosynthesis in Saccharomyces cerevisiae. Isolation and sequence of COQ3, the 3,4-dihydroxy-5-hexaprenylbenzoate methyltransferase gene. In The Journal of biological chemistry, 266, 16636-44. doi:. https://pubmed.ncbi.nlm.nih.gov/1885593/
6. Poon, W W, Barkovich, R J, Hsu, A Y, Myles, D C, Clarke, C F. . Yeast and rat Coq3 and Escherichia coli UbiG polypeptides catalyze both O-methyltransferase steps in coenzyme Q biosynthesis. In The Journal of biological chemistry, 274, 21665-72. doi:. https://pubmed.ncbi.nlm.nih.gov/10419476/
7. Avelange-Macherel, M H, Joyard, J. . Cloning and functional expression of AtCOQ3, the Arabidopsis homologue of the yeast COQ3 gene, encoding a methyltransferase from plant mitochondria involved in ubiquinone biosynthesis. In The Plant journal : for cell and molecular biology, 14, 203-13. doi:. https://pubmed.ncbi.nlm.nih.gov/9628017/
8. Hsu, A Y, Do, T Q, Lee, P T, Clarke, C F. . Genetic evidence for a multi-subunit complex in the O-methyltransferase steps of coenzyme Q biosynthesis. In Biochimica et biophysica acta, 1484, 287-97. doi:. https://pubmed.ncbi.nlm.nih.gov/10760477/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen