C57BL/6JCya-L1td1em1/Cya
Common Name:
L1td1-KO
Product ID:
S-KO-20018
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
L1td1-KO
Strain ID
KOCMP-381591-L1td1-B6J-VB
Gene Name
Product ID
S-KO-20018
Gene Alias
ECAT11
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
4
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-L1td1em1/Cya mice (Catalog S-KO-20018) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000154279
NCBI RefSeq
NM_001081202
Target Region
Exon 4~5
Size of Effective Region
~3.1 kb
Detailed Document
Overview of Gene Research
L1TD1, also known as FLJ10884 or ECAT11, is a cytoplasmic RNA-binding protein. It is specifically expressed in pluripotent stem cells and is essential for maintaining stemness in human cells. L1TD1 is the only known protein-coding gene domesticated from a LINE-1 (L1) retroelement. It plays roles in RNA splicing, translation, protein traffic, and degradation, and is part of the pluripotency interactome network of OCT4, SOX2, and NANOG, bridging nuclear and cytoplasmic regulation [2,3].
In medulloblastoma, high L1TD1 expression is correlated with poor prognosis. Depletion of L1TD1 protein levels by targeted gene silencing significantly reduces medulloblastoma cell viability, inhibits cell proliferation, induces apoptosis, downregulates neural stem cell markers (CD133 and Nestin), inhibits neurosphere generation, and sensitizes cells to chemotherapeutic agents [5]. In contrast, in colon cancer, increased L1TD1 expression is a positive prognostic marker associated with longer disease-free survival, and it shows a lack of co-expression with its interaction partners compared to medulloblastoma [1]. In non-small cell lung cancers (NSCLC), L1TD1 is tumor-specifically methylated, and its DNA methylation is involved in transcriptional regulation. Overexpression of L1TD1 in NSCLC cells reduces cell growth, proliferation, and viability both in vitro and in vivo [4].
In conclusion, L1TD1 has diverse functions in maintaining pluripotency and is involved in multiple disease conditions. The study of L1TD1 in disease models such as medulloblastoma, colon cancer, and NSCLC reveals its complex role in tumorigenesis, prognosis, and response to treatment, highlighting its potential as a prognostic marker and therapeutic target.
References:
1. Chakroborty, Deepankar, Emani, Maheswara Reddy, Klén, Riku, Lahesmaa, Riitta, Elo, Laura L. 2019. L1TD1 - a prognostic marker for colon cancer. In BMC cancer, 19, 727. doi:10.1186/s12885-019-5952-2. https://pubmed.ncbi.nlm.nih.gov/31337362/
2. Jin, Sang Woo, Seong, Youngmo, Yoon, Dayoung, Kwon, Young-Soo, Song, Hoseok. . Dissolution of ribonucleoprotein condensates by the embryonic stem cell protein L1TD1. In Nucleic acids research, 52, 3310-3326. doi:10.1093/nar/gkad1244. https://pubmed.ncbi.nlm.nih.gov/38165001/
3. Emani, Maheswara Reddy, Närvä, Elisa, Stubb, Aki, Elo, Laura L, Lahesmaa, Riitta. 2015. The L1TD1 protein interactome reveals the importance of post-transcriptional regulation in human pluripotency. In Stem cell reports, 4, 519-28. doi:10.1016/j.stemcr.2015.01.014. https://pubmed.ncbi.nlm.nih.gov/25702638/
4. Altenberger, Corinna, Heller, Gerwin, Ziegler, Barbara, Zielinski, Christoph C, Zöchbauer-Müller, Sabine. 2017. SPAG6 and L1TD1 are transcriptionally regulated by DNA methylation in non-small cell lung cancers. In Molecular cancer, 16, 1. doi:10.1186/s12943-016-0568-5. https://pubmed.ncbi.nlm.nih.gov/28093071/
5. Santos, Márcia Cristina Teixeira, Silva, Patrícia Benites Gonçalves, Rodini, Carolina Oliveira, Moreira, José C F, Okamoto, Oswaldo Keith. 2015. Embryonic Stem Cell-Related Protein L1TD1 Is Required for Cell Viability, Neurosphere Formation, and Chemoresistance in Medulloblastoma. In Stem cells and development, 24, 2700-8. doi:10.1089/scd.2015.0052. https://pubmed.ncbi.nlm.nih.gov/26159230/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen