C57BL/6JCya-Zmynd8em1/Cya
Common Name
Zmynd8-KO
Product ID
S-KO-20228
Backgroud
C57BL/6JCya
Strain ID
KOCMP-228880-Zmynd8-B6J-VB
When using this mouse strain in a publication, please cite “Zmynd8-KO Mouse (Catalog S-KO-20228) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Zmynd8-KO
Strain ID
KOCMP-228880-Zmynd8-B6J-VB
Gene Name
Product ID
S-KO-20228
Gene Alias
1110013E22Rik, 2010005I16Rik, 3632413B07Rik, Prkcbp1, RACK7
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 2
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000109269
NCBI RefSeq
NM_001291158
Target Region
Exon 4
Size of Effective Region
~1.3 kb
Overview of Gene Research
Zmynd8, also known as Zinc finger MYND-type containing 8, is a transcription factor, histone H3-interacting protein, and a putative chromatin reader/effector. It plays a crucial role in regulating transcription during normal cellular growth and is involved in various biological processes and pathways. Mutations and altered expression of Zmynd8 are associated with the development and progression of cancer [2].
In breast cancer, Zmynd8 was found to be upregulated in breast cancer stem cells (BCSCs). Gene-related functional studies in mouse models showed that Zmynd8 reduces ROS and iron to inhibit ferroptosis in aldehyde dehydrogenase-high (ALDHhi) BCSCs, leading to BCSC expansion and tumor initiation. This occurs through a two-fold post-translational regulation of nuclear factor erythroid 2-related factor 2 (NRF2), increasing NRF2 protein stability and enhancing antioxidant gene transcription [1]. In acute myeloid leukemia (AML), Zmynd8 directly activates IRF8 in parallel with the MYC proto-oncogene through their lineage-specific enhancers, and is essential for AML proliferation in vitro and in vivo [3].
In conclusion, Zmynd8 has diverse essential biological functions. Its role in promoting cancer development, such as in breast cancer and AML, has been revealed through model-based research, especially KO/CKO mouse models. Understanding Zmynd8's functions contributes to a deeper understanding of cancer-related biological processes and may provide new therapeutic targets for these diseases.
References：
1. Luo, Maowu, Bao, Lei, Xue, Yuanyuan, Wang, Yingfei, Luo, Weibo. 2024. ZMYND8 protects breast cancer stem cells against oxidative stress and ferroptosis through activation of NRF2. In The Journal of clinical investigation, 134, . doi:10.1172/JCI171166. https://pubmed.ncbi.nlm.nih.gov/38488001/
2. Chen, Yun, Tsai, Ya-Hui, Tseng, Sheng-Hong. 2021. Regulation of ZMYND8 to Treat Cancer. In Molecules (Basel, Switzerland), 26, . doi:10.3390/molecules26041083. https://pubmed.ncbi.nlm.nih.gov/33670804/
3. Cao, Zhendong, Budinich, Krista A, Huang, Hua, Berger, Shelley L, Shi, Junwei. 2021. ZMYND8-regulated IRF8 transcription axis is an acute myeloid leukemia dependency. In Molecular cell, 81, 3604-3622.e10. doi:10.1016/j.molcel.2021.07.018. https://pubmed.ncbi.nlm.nih.gov/34358447/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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