C57BL/6JCya-Cers4em1/Cya
Common Name
Cers4-KO
Product ID
S-KO-20311
Backgroud
C57BL/6JCya
Strain ID
KOCMP-67260-Cers4-B6J-VB
Status
When using this mouse strain in a publication, please cite “Cers4-KO Mouse (Catalog S-KO-20311) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Cers4-KO
Strain ID
KOCMP-67260-Cers4-B6J-VB
Gene Name
Product ID
S-KO-20311
Gene Alias
Trh1, Lass4, 2900019C14Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 8
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000008350
NCBI RefSeq
NM_026058
Target Region
Exon 3
Size of Effective Region
~0.8 kb
Overview of Gene Research
Cers4, or ceramide synthase 4, is an enzyme in the sphingolipid pathway, catalyzing de novo ceramide synthesis [1]. Sphingolipid metabolism is closely related to various biological processes and disease states. Cers4 is involved in multiple pathways, and its function is crucial in maintaining normal physiological functions and may be related to disease development. Genetic models, such as knockout (KO) mice, are valuable for studying its role.
In a T-cell-specific CerS4 depletion model (CerS4 LCK/Cre), mice frequently suffered from pancolitis and developed more colon tumors, while CerS4 deficiency in epithelial cells (CerS4 Vil/Cre) led to smaller colon tumors [2]. In high-fat-diet-fed C57BL/6J mice, downregulation of CerS4 using shRNA-mediated hydrodynamic gene delivery reduced specific liver ceramides, promoted glycogen accumulation, enhanced insulin sensitivity, and mitigated hepatic insulin resistance [3].
In conclusion, Cers4 plays a significant role in inflammation, tumor development, and metabolic regulation. The use of KO and conditional knockout (CKO) mouse models has revealed its complex functions in colitis-associated cancer and hepatic insulin resistance, providing insights into potential therapeutic targets for related diseases.
References：
1. Luo, Yuhong, Yang, Shoumei, Wu, Xuan, Liu, Weiwei, Gonzalez, Frank J. 2021. Intestinal MYC modulates obesity-related metabolic dysfunction. In Nature metabolism, 3, 923-939. doi:10.1038/s42255-021-00421-8. https://pubmed.ncbi.nlm.nih.gov/34211180/
2. El-Hindi, Khadija, Brachtendorf, Sebastian, Hartel, Jennifer Christina, Utermöhlen, Olaf, Grösch, Sabine. 2022. T-Cell-Specific CerS4 Depletion Prolonged Inflammation and Enhanced Tumor Burden in the AOM/DSS-Induced CAC Model. In International journal of molecular sciences, 23, . doi:10.3390/ijms23031866. https://pubmed.ncbi.nlm.nih.gov/35163788/
3. Roszczyc-Owsiejczuk, Kamila, Zabielski, Piotr, Imierska, Monika, Sadowska, Patrycja, Błachnio-Zabielska, Agnieszka. . Downregulation of CerS4 Instead of CerS2 in Liver Effectively Alleviates Hepatic Insulin Resistance in HFD Male Mice. In Endocrinology, 165, . doi:10.1210/endocr/bqae118. https://pubmed.ncbi.nlm.nih.gov/39233348/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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