C57BL/6JCya-Gp2em1/Cya
Common Name:
Gp2-KO
Product ID:
S-KO-20370
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Gp2-KO
Strain ID
KOCMP-67133-Gp2-B6J-VA
Gene Name
Product ID
S-KO-20370
Gene Alias
2310037I18Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gp2em1/Cya mice (Catalog S-KO-20370) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000033255
NCBI RefSeq
NM_025989
Target Region
Exon 4~5
Size of Effective Region
~2.0 kb
Detailed Document
Overview of Gene Research
Gp2, or glycoprotein 2, has diverse functions. Initially identified as a major membranous component in pancreatic acinar cells, it also plays a crucial role in mucosal immunology. In the intestine, it serves as an uptake receptor for certain commensal and pathogenic bacteria by interacting with FimH, facilitating mucosal immune responses [3]. It's also associated with regulated exocrine secretion, where it may be involved in membrane sorting, inactivation of zymogen granule membranes, and their trafficking [4].
In the context of human pluripotent stem cell-derived pancreatic progenitors (PPs), GP2 has emerged as a specific cell surface marker. GP2-enriched PPs can give rise to all pancreatic cells in vivo, including functional beta-like cells, and eliminate the risk of teratoma formation, which is significant for type 1 diabetes treatment [1]. Moreover, a novel PP differentiation platform based on increasing Glycoprotein-2 expression was introduced, with GP2-high PPs allowing generation of pancreatic acinar-like organoids, and GP2 expression levels at the PP stage being associated with varying pancreatic lineage potential [2].
In summary, Gp2 has key functions in mucosal immunity and pancreatic cell development. Its role in reducing teratoma risk in pancreatic progenitor-based therapies for type 1 diabetes, and its association with pancreatic lineage potential, underscore its importance in these disease-relevant biological processes.
References:
1. Aghazadeh, Yasaman, Sarangi, Farida, Poon, Frankie, Nunes, Sara S, Nostro, M Cristina. 2022. GP2-enriched pancreatic progenitors give rise to functional beta cells in vivo and eliminate the risk of teratoma formation. In Stem cell reports, 17, 964-978. doi:10.1016/j.stemcr.2022.03.004. https://pubmed.ncbi.nlm.nih.gov/35364010/
2. Merz, Sarah, Breunig, Markus, Melzer, Michael Karl, Hohwieler, Meike, Kleger, Alexander. 2023. Single-cell profiling of GP2-enriched pancreatic progenitors to simultaneously create acinar, ductal, and endocrine organoids. In Theranostics, 13, 1949-1973. doi:10.7150/thno.78323. https://pubmed.ncbi.nlm.nih.gov/37064874/
3. Ohno, Hiroshi, Hase, Koji. . Glycoprotein 2 (GP2): grabbing the FimH bacteria into M cells for mucosal immunity. In Gut microbes, 1, 407-10. doi:10.4161/gmic.1.6.14078. https://pubmed.ncbi.nlm.nih.gov/21468225/
4. Scheele, G A, Fukuoka, S, Freedman, S D. . Role of the GP2/THP family of GPI-anchored proteins in membrane trafficking during regulated exocrine secretion. In Pancreas, 9, 139-49. doi:. https://pubmed.ncbi.nlm.nih.gov/8190715/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen