C57BL/6JCya-Itga2em1/Cya
Common Name:
Itga2-KO
Product ID:
S-KO-20412
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Itga2-KO
Strain ID
KOCMP-16398-Itga2-B6J-VB
Gene Name
Product ID
S-KO-20412
Gene Alias
CD49B; DX5; GPIa
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
13
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Itga2em1/Cya mice (Catalog S-KO-20412) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000056117
NCBI RefSeq
NM_008396
Target Region
Exon 2
Size of Effective Region
~1.7 kb
Detailed Document
Overview of Gene Research
Itga2, also known as CD49b or VLA-2, is the alpha subunit of the transmembrane collagen receptor integrin alpha-2/beta-1. It plays crucial roles in cell adhesion, and is involved in regulating the DNA damage response (DDR) pathway [1]. It has been associated with various biological processes relevant to cancer, such as cell invasion, migration, and immune regulation [2-10]. Genetic models, like gene knockout (KO) or conditional knockout (CKO) mouse models, could potentially further elucidate its functions.
In pancreatic cancer, ITGA2 overexpression inhibits DNA repair via restraining the recruitment of DNA-PKcs to Ku70/80 heterodimer in the non-homologous end joining (NHEJ) pathway, conferring sensitivity to radiotherapy [1]. In glioblastoma, ITGA2 antibody blockade significantly impedes cell migration [2]. In colorectal cancer, ITGA2 is associated with 5-fluorouracil resistance, and knocking down ITGA2 can restore sensitivity to 5-FU [3]. In pancreatic cancer, ITGA2 can also activate the cytosolic DNA-sensing pathway and promote STING expression by inducing DNMT1 degradation [4]. In prostate cancer, downregulation or genomic loss of ITGA2 is associated with tumor progression and EMT induction [5]. In esophageal squamous cell carcinoma, ITGA2 promotes proliferation, invasion, migration, and EMT via the FAK/AKT signaling pathway [6]. In intrahepatic cholangiocarcinoma, elevated ITGA2 expression promotes collagen type I-induced clonogenic growth [7]. In thyroid carcinoma, hypomethylation-mediated overexpression of ITGA2 stimulates cell invasion and migration [8]. In glioma, ITGA2 promotes glioma stem cell (GSC) invasion and EMT by activating STAT3 phosphorylation [9].
In conclusion, Itga2 is a key gene involved in multiple biological processes, especially in cancer-related pathways such as DNA repair, cell invasion, migration, and immune regulation. Research using KO or CKO mouse models (not explicitly detailed in the provided references but potentially valuable) could further clarify its functions. The studies on Itga2 contribute to understanding cancer mechanisms, providing potential therapeutic targets for various cancers including pancreatic, glioblastoma, colorectal, prostate, esophageal, intrahepatic cholangiocarcinoma, thyroid, and glioma cancers.
References:
1. Zhou, Chen, Li, Shoukang, Bin, Kaijian, Wu, Heshui, Zhou, Yingke. 2022. ITGA2 overexpression inhibits DNA repair and confers sensitivity to radiotherapies in pancreatic cancer. In Cancer letters, 547, 215855. doi:10.1016/j.canlet.2022.215855. https://pubmed.ncbi.nlm.nih.gov/35998796/
2. Guo, Peng, Moses-Gardner, Alexander, Huang, Jing, Smith, Edward R, Moses, Marsha A. 2019. ITGA2 as a potential nanotherapeutic target for glioblastoma. In Scientific reports, 9, 6195. doi:10.1038/s41598-019-42643-7. https://pubmed.ncbi.nlm.nih.gov/30996239/
3. Qin, Jian, Hu, Shangshang, Lou, Jinwei, Pan, Yuqin, Wang, Shukui. 2024. Selumetinib overcomes ITGA2-induced 5-fluorouracil resistance in colorectal cancer. In International immunopharmacology, 137, 112487. doi:10.1016/j.intimp.2024.112487. https://pubmed.ncbi.nlm.nih.gov/38889513/
4. Meng, Junpeng, Cai, Hongkun, Sun, Yan, Wu, Heshui, Ren, Dianyun. 2022. ITGA2 induces STING expression in pancreatic cancer by inducing DNMT1 degradation. In Cellular oncology (Dordrecht, Netherlands), 45, 1421-1434. doi:10.1007/s13402-022-00731-3. https://pubmed.ncbi.nlm.nih.gov/36331797/
5. Cruz, Sara P, Zhang, Qin, Devarajan, Raman, Wei, Gong-Hong, Manninen, Aki. 2024. Dampened Regulatory Circuitry of TEAD1/ITGA1/ITGA2 Promotes TGFβ1 Signaling to Orchestrate Prostate Cancer Progression. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 11, e2305547. doi:10.1002/advs.202305547. https://pubmed.ncbi.nlm.nih.gov/38169150/
6. Huang, Wei, Zhu, Ju, Shi, Haoming, Wu, Qingchen, Zhang, Cheng. 2021. ITGA2 Overexpression Promotes Esophageal Squamous Cell Carcinoma Aggression via FAK/AKT Signaling Pathway. In OncoTargets and therapy, 14, 3583-3596. doi:10.2147/OTT.S302028. https://pubmed.ncbi.nlm.nih.gov/34113124/
7. Rattanasinchai, Chotirat, Navasumrit, Panida, Ruchirawat, Mathuros. 2022. Elevated ITGA2 expression promotes collagen type I-induced clonogenic growth of intrahepatic cholangiocarcinoma. In Scientific reports, 12, 22429. doi:10.1038/s41598-022-26747-1. https://pubmed.ncbi.nlm.nih.gov/36575207/
8. Chen, Hong, Zhang, Chunying, Li, Yanbing, Chen, Chunyou. 2022. Hypomethylation-mediated overexpression of ITGA2 stimulates cell invasion and migration of thyroid carcinoma. In Histology and histopathology, 38, 787-796. doi:10.14670/HH-18-552. https://pubmed.ncbi.nlm.nih.gov/36420922/
9. Zhang, Jin, Li, Ruinan, Zhang, Haibin, Wang, Shanshan, Zhao, Yuanli. . ITGA2 as a prognostic factor of glioma promotes GSCs invasion and EMT by activating STAT3 phosphorylation. In Carcinogenesis, 45, 235-246. doi:10.1093/carcin/bgad096. https://pubmed.ncbi.nlm.nih.gov/38142122/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen