The TAG stop codon was replaced with "P2A-CreERT2" cassette. CreERT2 recombinase is expressed under the regulatory control of Ace2 gene elements.

The TAA stop codon was replaced with the "P2A-CreERT2" cassette. CreERT2 recombinase is expressed under the regulatory control of Adgre1 gene elements. This model is a Tamoxifen-inducible Cre mouse, and when crossed with mice containing loxP sites, the offspring mice are expected to undergo sequence recombination between loxP sites mediated by Cre recombinase in macrophages following Tamoxifen induction.

The “Cre-P2A” cassette will be inserted upstream of the ATG start codon.

The ADIPOQ gene-encoded adiponectin is a protein hormone produced exclusively by adipocytes (fat cells). It is transported through the bloodstream to muscle and liver cells. In this model, the P2A-iCre gene expression element is inserted into the mouse endogenous Adipoq gene at the stop codon. Its specific expression is regulated by the mouse Adipoq gene. When this strain is crossed with mice containing loxP sites, sequence recombination mediated by the Cre recombinase between loxP sites can occur in the white adipose tissue (WAT) and brown adipose tissue (BAT) of its offspring.

The AGRP gene encodes Agouti-related protein (AgRP), a neuropeptide produced by AgRP/NPY neurons in the brain, primarily expressed in the arcuate nucleus of the hypothalamus, kidneys, and adrenal glands. Studies have shown that AGRP gene expression significantly increases during starvation and rapidly decreases after feeding. AgRP is synthesized exclusively in the ventromedial part of the arcuate nucleus in cells containing neuropeptide Y (NPY) and is co-expressed with NPY, playing a role in increasing appetite, reducing metabolism, and decreasing energy expenditure. The Agrp-IRES-CreERT2-P2A-tdTomato mouse model was created by integrating the IRES-CreERT2-P2A-tdTomato gene expression cassette into the mouse Agrp gene. Under the control of the mouse endogenous Agrp gene regulatory elements, this mouse expresses tamoxifen-inducible CreERT2 recombinase. Additionally, the expression cassette includes a red fluorescent protein (tdTomato) expression element for lineage tracing of Agrp-positive cells. When Agrp-IRES-CreERT2-P2A-tdTomato mice are crossed with mice containing loxP sites, tamoxifen induction can trigger Cre recombinase-mediated sequence recombination between loxP sites in AgRP-positive neurons of the offspring.

The TGA stop codon was replaced with "P2A-CreERT2" cassette.

The TAA stop codon was replaced with the "P2A-EGFP-T2A-iCre" cassette. Cre recombinase and enhanced green fluorescent protein (EGFP) are expressed under the regulatory control of Alas2 gene elements, without impacting the endogenous expression of the Alas2 gene. In addition to studies of Cre recombinase-mediated gene recombination, this model can also be used for EGFP fluorescent protein tracing studies.

The TGA stop codon was replaced by "P2A-Cre". A synonymous mutation p.R894=(CGG to CGC) and an additional mutation c.*3C>G in 3'UTR were also introduced to prevent the binding and re-cutting of the sequence by gRNA after homology-directed repair.

The TGA stop codon was replaced with the "P2A-CreERT2-WPRE-BGH pA" cassette. CreERT2 recombinase is expressed under the regulatory control of Aqp5 gene elements. This model is a Tamoxifen-inducible Cre mouse, and when crossed with mice containing loxP sites, the offspring mice are expected to undergo sequence recombination between loxP sites mediated by Cre recombinase in alveolar type I cells, salivary gland alveolar cells, and gastrointestinal stem cells following Tamoxifen induction.

The TGA stop codon was replaced by“P2A-CreERT2”cassette, thus the expression of Cre was under control of the mouse Bcl6 gene regulatory element.