Adipoq-iCre Mouse
Product Name
Adipoq-iCre Mouse
Product ID
C001529
Strain Name
C57BL/6JCya-Adipoqem1(P2A-iCre)/Cya
Backgroud
C57BL/6JCya
Examples of Expressing Tissues/Cells
Adipocytes
Status
When using this mouse strain in a publication, please cite “Adipoq-iCre Mouse (Catalog C001529) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Gene Name
Adipoq
Gene Alias
Ad, APN, Acdc, Adid, apM1, 30kDa, GBP28, adipo, Acrp30
NCBI ID
Chromosome
Chr 16 (Mouse)
MGI ID
Datasheet
Strain Description
The ADIPOQ gene-encoded adiponectin is a protein hormone produced exclusively by adipocytes (fat cells). It is transported through the bloodstream to muscle and liver cells. Adiponectin regulates various pathways related to fat storage and metabolism, including the modulation of blood glucose levels, fatty acid breakdown, brown adipocyte differentiation, and negative regulation of gluconeogenesis. By increasing insulin sensitivity and promoting fatty acid breakdown, adiponectin plays a crucial role in regulating glucose and fat metabolism. Additionally, it exhibits direct anti-diabetic, anti-atherosclerotic, and anti-inflammatory activities [1-2]. The mutation of the ADIPOQ gene is associated with adiponectin deficiency syndrome. Although the ADIPOQ gene is expressed predominantly (or almost exclusively) in adipose tissue, adiponectin, as a secreted hormone, circulates via the bloodstream and is widely distributed in various tissues and organs, including skeletal muscle, liver, intestine, male reproductive glands, and brain, where it exerts its physiological effects through specific receptors (such as AdipoR1 and AdipoR2) [3-4].
The Adipoq-iCre mice are constructed by inserting a codon-improved Cre recombinase (iCre) element into the endogenous Adipoq gene of mice. The expression pattern of iCre recombinase is similar to the endogenous gene. When this strain is crossed with mice containing loxP sites, sequence recombination mediated by the Cre recombinase between loxP sites can occur in the white adipose tissue (WAT) and brown adipose tissue (BAT) of its offspring.
Reference
Maeda K, Okubo K, Shimomura I, Funahashi T, Matsuzawa Y, Matsubara K. cDNA cloning and expression of a novel adipose specific collagen-like factor, apM1 (AdiPose Most abundant Gene transcript 1). Biochem Biophys Res Commun. 1996 Apr 16;221(2):286-9.
Martinez-Huenchullan SF, Tam CS, Ban LA, Ehrenfeld-Slater P, Mclennan SV, Twigg SM. Skeletal muscle adiponectin induction in obesity and exercise. Metabolism. 2020 Jan;102:154008.
Oliveira CS, Giuffrida FM, Crispim F, Saddi-Rosa P, Reis AF. ADIPOQ and adiponectin: the common ground of hyperglycemia and coronary artery disease? Arq Bras Endocrinol Metabol. 2011 Oct;55(7):446-54.
Spranger J, Verma S, Göhring I, Bobbert T, Seifert J, Sindler AL, Pfeiffer A, Hileman SM, Tschöp M, Banks WA. Adiponectin does not cross the blood-brain barrier but modifies cytokine expression of brain endothelial cells. Diabetes. 2006 Jan;55(1):141-7.
Strain Strategy
The stop codon was replaced with the P2A-iCre cassette.
Figure 1. Diagram of the gene editing strategy for the generation of Adipoq-iCre mice.
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