The AGRP gene encodes Agouti-related protein (AgRP), a neuropeptide produced by AgRP/NPY neurons in the brain, primarily expressed in the arcuate nucleus of the hypothalamus, kidneys, and adrenal glands. Studies have shown that AGRP gene expression significantly increases during starvation and rapidly decreases after feeding. AgRP is synthesized exclusively in the ventromedial part of the arcuate nucleus in cells containing neuropeptide Y (NPY) and is co-expressed with NPY, playing a role in increasing appetite, reducing metabolism, and decreasing energy expenditure. The Agrp-IRES-CreERT2-P2A-tdTomato mouse model was created by integrating the IRES-CreERT2-P2A-tdTomato gene expression cassette into the mouse Agrp gene. Under the control of the mouse endogenous Agrp gene regulatory elements, this mouse expresses tamoxifen-inducible CreERT2 recombinase. Additionally, the expression cassette includes a red fluorescent protein (tdTomato) expression element for lineage tracing of Agrp-positive cells. When Agrp-IRES-CreERT2-P2A-tdTomato mice are crossed with mice containing loxP sites, tamoxifen induction can trigger Cre recombinase-mediated sequence recombination between loxP sites in AgRP-positive neurons of the offspring.