Serpina1 (a-e) KO mice are gene knockout (KO) models created by gene editing technology to knock out the protein-coding sequences of the Serpina1 (a-e) genes (homologous to the human SERPINA1 gene) in mice. Preliminary data indicate almost no expression of the Serpina1 gene in the liver of these mice, with no significant pulmonary abnormalities observed at 8 weeks of age. Serpina1 (a-e) KO mice can be used to study the pathogenic mechanisms of emphysema and chronic obstructive pulmonary disease (COPD), cirrhosis, and hepatocellular carcinoma, as well as to develop related therapeutic approaches.