FVB-MMTV-PyMT Mouse
Product Name
FVB-MMTV-PyMT Mouse
Product ID
C001895
Strain Name
FVB/NJCya-Tg(MMTV-PyVT)/Cya
Backgroud
FVB/NJCya
Status
When using this mouse strain in a publication, please cite “FVB-MMTV-PyMT Mouse (Catalog C001895) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Strain Description
The Mouse Mammary Tumor Virus (MMTV) is a retrovirus transmitted through milk and belongs to the betaretrovirus class. This virus was discovered during the study of inbred mouse strains with a high incidence of mammary tumors and can cause breast cancer and lymphoma in mice. It is the most studied oncogenic virus, causing about 95% of mouse breast cancer. As an endogenously encoded β-retrovirus, MMTV is transmitted as an exogenous pathogen through milk, using breast milk to vertically transmit from the mother to the pups, integrating as a DNA provirus in the DNA of milk lymphocytes. During puberty, the virus enters the mammary gland with migrating lymphocytes and infects proliferating mammary epithelial cells. As a retrovirus, MMTV can insert its viral genome into the host genome [1-2]. The long terminal repeat sequence (LTR) of MMTV contains a glucocorticoid response element that acts as a promoter during transcription. Utilizing its viral tissue-specific promoter and enhancer functions, the related structure of this virus is also used to drive the specific expression of certain genes in tissues such as mouse mammary glands, for the construction of mammary tumor-like disease models or for conducting tissue-specific research related to the mammary glands [3-4].
The FVB-MMTV-PyMT mouse is a tumor research model, in which the cDNA sequence of the polyoma middle T antigen (PyVT/PyMT) from the polyoma virus is integrated into the mouse genome through transgenic technology. This is driven by the long terminal repeat sequence (LTR) of the mouse MMTV for its specific expression. The PyVT protein can be expressed in mammary epithelial cells, leading to widespread transformation of the mammary epithelium and rapid generation of multifocal breast cancer [5]. It has been reported that adenocarcinomas in MMTV-PyMT mice mainly occur in females, but can occasionally be observed in males. These adenocarcinomas are well differentiated, multifocal, and eventually involve the entire mammary fat pad [6]. Detection data show that female FVB-MMTV-PyMT mice can develop the disease as early as 8 weeks of age. The incidence rate is approximately 80% at 10 weeks of age, and lung metastasis occurs in the late stage. Compared with Cyagen's similar strains, MMTV-PyMT mice (C57BL/6JCya background, catalog number: C001212) and FVB;B6-MMTV-PyMT (F1) mice (catalog number: C001556), FVB-MMTV-PyMT mice with an FVB/NJCya background develop the disease earlier.
It should be noted that due to individual differences and environmental factors, the disease occurrence in this model may vary. The data in this strain datasheet were obtained under Cyagen's internal facility conditions and are for reference only. The actual disease occurrence in mice should prevail. Please use the model appropriately according to the actual situation.
Reference
Matsuzawa A, Nakano H, Yoshimoto T, Sayama K. Biology of mouse mammary tumor virus (MMTV). Cancer Lett. 1995 Mar 23;90(1):3-11.
Parisi F, Freer G, Mazzanti CM, Pistello M, Poli A. Mouse Mammary Tumor Virus (MMTV) and MMTV-like Viruses: An In-depth Look at a Controversial Issue. Viruses. 2022 May 6;14(5):977.
Truss M, Chalepakis G, Beato M. Interplay of steroid hormone receptors and transcription factors on the mouse mammary tumor virus promoter. J Steroid Biochem Mol Biol. 1992 Oct;43(5):365-78.
Wagner KU, Wall RJ, St-Onge L, Gruss P, Wynshaw-Boris A, Garrett L, Li M, Furth PA, Hennighausen L. Cre-mediated gene deletion in the mammary gland. Nucleic Acids Res. 1997 Nov 1;25(21):4323-30.
Guy CT, Cardiff RD, Muller WJ. Induction of mammary tumors by expression of polyomavirus middle T oncogene: a transgenic mouse model for metastatic disease. Mol Cell Biol. 1992 Mar;12(3):954-61.
Attalla S, Taifour T, Bui T, Muller W. Insights from transgenic mouse models of PyMT-induced breast cancer: recapitulating human breast cancer progression in vivo. Oncogene. 2021 Jan;40(3):475-491.
Strain Strategy
Figure 1. Gene editing strategy of FVB-MMTV-PyMT mice. Expression elements of the MMTV-driven PyVT cDNA sequence were transferred into mice using transgenic (TG) technology.
Application Area
Research on breast tumors;
Research related to the tumor microenvironment.
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