huCD86 Mouse
Product Name
huCD86 Mouse
Product ID
C002003
Strain Name
C57BL/6NCya-Cd86tm1(hCD86)/Cya
Backgroud
C57BL/6NCya
Status
When using this mouse strain in a publication, please cite “huCD86 Mouse (Catalog C002003) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Gene Name
CD86
Gene Alias
B70, B7-2, B7.2, LAB72, CD28LG2
NCBI ID
Chromosome
Chr 3 (Human)
MGI ID
Datasheet
Strain Description
The CD86 gene (Cluster of Differentiation 86) encodes a critical type I transmembrane glycoprotein that serves as a potent costimulatory ligand in the adaptive immune response. Primarily expressed on the surface of antigen-presenting cells (APCs)—including activated B cells, macrophages, and dendritic cells—the CD86 protein (also known as B7-2) acts as a ligand for two distinct receptors on T cells: the stimulatory receptor CD28 and the inhibitory receptor CTLA-4 [1]. Its primary function is to provide the "second signal" necessary for T cell activation, proliferation, and cytokine production following the initial recognition of an antigen. Beyond lymphoid tissues, CD86 labeling is a hallmark of activated myeloid lineages and is often used to characterize M1-polarized macrophages [2]. Dysregulation of the CD86 pathway is significantly associated with various autoimmune diseases, such as systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA), as well as the immune evasion mechanisms observed in several types of hematologic malignancies and solid tumors [3].
The huCD86 mouse model was generated by replacing the endogenous extracellular domain of mouse Cd86 with the extracellular domain of human CD86, while retaining the mouse signal peptide and aa.245~309. This model is suitable for the study of various autoimmune diseases—including systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA)—as well as multiple malignancies, and for the development of CD86-targeted therapeutics.
Reference
Barna G, Szalóki G, Márk Á, Hunyadi A, Kriston C. CD86, the double agent: Significance of CD86 expression in B-cell malignancies. Int J Cancer. 2025 Nov 1;157(9):1772-1780.
Smith TD, Tse MJ, Read EL, Liu WF. Regulation of macrophage polarization and plasticity by complex activation signals. Integr Biol (Camb). 2016 Sep 12;8(9):946-55.
Liu W, Yang Z, Chen Y, Yang H, Wan X, Zhou X, Liu R, Zhang Y. The Association Between CTLA-4, CD80/86, and CD28 Gene Polymorphisms and Rheumatoid Arthritis: An Original Study and Meta-Analysis. Front Med (Lausanne). 2021 Feb 2;8:598076.
Strain Strategy
The mouse Cd86 endogenous extracellular domain was replaced with the human CD86 extracellular domain. The murine signal peptide and aa.245~309 were preserved.
Figure 1. Gene editing strategy of huCD86 mice.
Application Area
Screening, development, and preclinical evaluation of CD86-targeted drugs;
Research on the various autoimmune diseases, such as systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA);
Research on the pathological mechanisms and treatment methods of malignant tumors.
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