C57BL/6JCya-Mettl1em1flox/Cya
Common Name:
Mettl1-flox
Product ID:
S-CKO-03718
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Mettl1-flox
Strain ID
CKOCMP-17299-Mettl1-B6J-VA
Gene Name
Product ID
S-CKO-03718
Gene Alias
2810012D02Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mettl1em1flox/Cya mice (Catalog S-CKO-03718) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000006915
NCBI RefSeq
NM_010792
Target Region
Exon 3~6
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
METTL1, the methyltransferase-like 1, is a key component of the tRNA N7-methylguanosine (m7G) methyltransferase complex along with WDR4. It catalyzes m7G modification of tRNAs, which is crucial for tRNA stability and function, and is involved in processes like mRNA translation [5,7,9]. This modification impacts various biological processes and is associated with multiple diseases, especially cancers [1,2,3,4,6,7,8].
Depletion of METTL1 in cancer cells leads to decreased abundance of m7G-modified tRNAs, altered cell cycle, and inhibited oncogenicity. For example, in hepatocellular carcinoma (HCC), METTL1 knockdown overrode lenvatinib resistance by impairing cell proliferation and promoting apoptosis. In bladder cancer, silencing METTL1 suppressed cell proliferation, migration, and invasion both in vitro and in vivo. In prostate cancer, METTL1 depletion increased intratumoural infiltration of pro-inflammatory immune cells [1,2,3,8]. In post-radiofrequency ablation recurrent HCC, heat-mediated METTL1 upregulation enhanced TGF-β2 translation to form an immunosuppressive environment. Interrupting the METTL1-TGF-β2-PMN-MDSC axis could prevent HCC recurrence [4].
In conclusion, METTL1-mediated m7G tRNA modification is essential in regulating various biological processes. Through gene knockout or knockdown studies in different cancer models, it has been shown to play a significant role in cancer development, drug resistance, and immunosuppression. Understanding METTL1-related mechanisms provides potential therapeutic targets for cancer treatment.
References:
1. Orellana, Esteban A, Liu, Qi, Yankova, Eliza, Tzelepis, Konstantinos, Gregory, Richard I. 2021. METTL1-mediated m7G modification of Arg-TCT tRNA drives oncogenic transformation. In Molecular cell, 81, 3323-3338.e14. doi:10.1016/j.molcel.2021.06.031. https://pubmed.ncbi.nlm.nih.gov/34352207/
2. Huang, Manling, Long, Jianting, Yao, Zhijia, Xu, Lixia, Kuang, Ming. . METTL1-Mediated m7G tRNA Modification Promotes Lenvatinib Resistance in Hepatocellular Carcinoma. In Cancer research, 83, 89-102. doi:10.1158/0008-5472.CAN-22-0963. https://pubmed.ncbi.nlm.nih.gov/36102722/
3. Ying, Xiaoling, Liu, Bixia, Yuan, Zusen, Luo, Junhang, Ji, Weidong. . METTL1-m7 G-EGFR/EFEMP1 axis promotes the bladder cancer development. In Clinical and translational medicine, 11, e675. doi:10.1002/ctm2.675. https://pubmed.ncbi.nlm.nih.gov/34936728/
4. Zeng, Xuezhen, Liao, Guanrui, Li, Shumin, Chen, Shuling, Kuang, Ming. 2022. Eliminating METTL1-mediated accumulation of PMN-MDSCs prevents hepatocellular carcinoma recurrence after radiofrequency ablation. In Hepatology (Baltimore, Md.), 77, 1122-1138. doi:10.1002/hep.32585. https://pubmed.ncbi.nlm.nih.gov/35598182/
5. Ruiz-Arroyo, Victor M, Raj, Rishi, Babu, Kesavan, Roberts, Paul H, Nam, Yunsun. 2023. Structures and mechanisms of tRNA methylation by METTL1-WDR4. In Nature, 613, 383-390. doi:10.1038/s41586-022-05565-5. https://pubmed.ncbi.nlm.nih.gov/36599982/
6. Zhang, Mingpeng, Kan, Duo, Zhang, Boya, Niu, Yuanjie, Shang, Zhiqun. 2023. P300/SP1 complex mediating elevated METTL1 regulates CDK14 mRNA stability via internal m7G modification in CRPC. In Journal of experimental & clinical cancer research : CR, 42, 215. doi:10.1186/s13046-023-02777-z. https://pubmed.ncbi.nlm.nih.gov/37599359/
7. Cheng, Wenli, Gao, Aili, Lin, Hui, Zhang, Wenjuan. 2022. Novel roles of METTL1/WDR4 in tumor via m7G methylation. In Molecular therapy oncolytics, 26, 27-34. doi:10.1016/j.omto.2022.05.009. https://pubmed.ncbi.nlm.nih.gov/35784404/
8. García-Vílchez, Raquel, Añazco-Guenkova, Ana M, Dietmann, Sabine, Carracedo, Arkaitz, Blanco, Sandra. 2023. METTL1 promotes tumorigenesis through tRNA-derived fragment biogenesis in prostate cancer. In Molecular cancer, 22, 119. doi:10.1186/s12943-023-01809-8. https://pubmed.ncbi.nlm.nih.gov/37516825/
9. Li, Jiazhi, Wang, Longfei, Hahn, Quentin, Fischer, Eric S, Gregory, Richard I. 2023. Structural basis of regulated m7G tRNA modification by METTL1-WDR4. In Nature, 613, 391-397. doi:10.1038/s41586-022-05566-4. https://pubmed.ncbi.nlm.nih.gov/36599985/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen