C57BL/6JCya-Nadkem1flox/Cya
Common Name:
Nadk-flox
Product ID:
S-CKO-04535
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Nadk-flox
Strain ID
CKOCMP-192185-Nadk-B6J-VA
Gene Name
Product ID
S-CKO-04535
Gene Alias
4432404C02Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Nadkem1flox/Cya mice (Catalog S-CKO-04535) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000105613
NCBI RefSeq
NM_001159637
Target Region
Exon 4
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Nadk, short for nicotinamide adenine dinucleotide (NAD+) kinase, is the sole enzyme that phosphorylates NAD+ to produce nicotinamide adenine dinucleotide phosphate (NADP), a crucial co-factor for lipid and redox homeostasis [1,2,3,4,7]. The NADK genes and the NADP(H)-producing mechanism are evolutionarily conserved among archaea, bacteria, plants, and mammals [1]. NADK is involved in multiple biological processes and has significant pathophysiological importance [1]. Genetic models have been valuable in validating its essential role in cellular redox homeostasis and metabolism in multicellular organisms [1].
In breast cancer, NADK-mediated de novo NADP(H) synthesis is essential for metastasis. Metastatic signals epigenetically up-regulate NADK, expanding the NADP(H) pools and enabling cells to adapt to the metastatic cascade [2]. In pancreatic ductal adenocarcinoma (PDAC), oncogenic KRAS promotes PKC-mediated NADK phosphorylation and hyperactivation, sustaining NADP+ and NADPH levels [3]. In the razor clam, ScNADK is involved in proline synthesis under high-salinity stress, enhancing the clam's ability to tolerate salinity variations [4]. In lung adenocarcinoma (LUAD), knockdown of NADK promotes ferroptosis by regulating the NADPH/FSP1 axis, as NADK is over-expressed in LUAD patients [5]. In non-small cell lung carcinoma, NUAK1-mediated phosphorylation of NADK at serine 64 mitigates osimertinib-induced ROS accumulation, leading to osimertinib resistance [6].
In conclusion, Nadk plays essential roles in maintaining cellular redox and metabolic homeostasis. Through model-based research, it has been shown to be involved in various disease conditions such as cancer metastasis, cancer cell survival under stress, and osmoregulation in organisms. The study of Nadk using gene knockout or knockdown models has provided insights into its role in these specific biological processes and disease areas, suggesting it could be a potential therapeutic target for related diseases.
References:
1. Oka, Shin-Ichi, Titus, Allen Sam, Zablocki, Daniela, Sadoshima, Junichi. 2022. Molecular properties and regulation of NAD+ kinase (NADK). In Redox biology, 59, 102561. doi:10.1016/j.redox.2022.102561. https://pubmed.ncbi.nlm.nih.gov/36512915/
2. Ilter, Didem, Drapela, Stanislav, Schild, Tanya, McReynolds, Melanie R, Gomes, Ana P. 2023. NADK-mediated de novo NADP(H) synthesis is a metabolic adaptation essential for breast cancer metastasis. In Redox biology, 61, 102627. doi:10.1016/j.redox.2023.102627. https://pubmed.ncbi.nlm.nih.gov/36841051/
3. Schild, Tanya, McReynolds, Melanie R, Shea, Christie, Gomes, Ana P, Blenis, John. . NADK is activated by oncogenic signaling to sustain pancreatic ductal adenocarcinoma. In Cell reports, 35, 109238. doi:10.1016/j.celrep.2021.109238. https://pubmed.ncbi.nlm.nih.gov/34133937/
4. Liu, Ruiqi, Deng, Min, Zhang, Na, Jia, Liang, Niu, Donghong. 2024. NADK-mediated proline synthesis enhances high-salinity tolerance in the razor clam. In Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 291, 111610. doi:10.1016/j.cbpa.2024.111610. https://pubmed.ncbi.nlm.nih.gov/38408517/
5. Meng, Xiangpeng, Peng, Fang, Yu, Shijie, Wang, Wenchi, Shao, Shujuan. 2024. Knockdown of NADK promotes LUAD ferroptosis via NADPH/FSP1 axis. In Journal of cancer research and clinical oncology, 150, 228. doi:10.1007/s00432-024-05752-z. https://pubmed.ncbi.nlm.nih.gov/38700533/
6. Lin, Wei, Wang, Na, Wu, Shihao, Liao, Yongde, Meng, Yunchong. . NUAK1-Mediated Phosphorylation of NADK Mitigates ROS Accumulation to Promote Osimertinib Resistance in Non-Small Cell Lung Carcinoma. In Cancer research, 84, 4081-4098. doi:10.1158/0008-5472.CAN-24-0249. https://pubmed.ncbi.nlm.nih.gov/39159134/
7. Petrelli, R, Felczak, K, Cappellacci, L. . NMN/NaMN adenylyltransferase (NMNAT) and NAD kinase (NADK) inhibitors: chemistry and potential therapeutic applications. In Current medicinal chemistry, 18, 1973-92. doi:. https://pubmed.ncbi.nlm.nih.gov/21517775/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen