C57BL/6JCya-Ptprz1em1flox/Cya
Common Name:
Ptprz1-flox
Product ID:
S-CKO-04616
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Ptprz1-flox
Strain ID
CKOCMP-19283-Ptprz1-B6J-VA
Gene Name
Product ID
S-CKO-04616
Gene Alias
DSD-1-PG; PTPbeta; PTPzeta; Ptprz; Ptpz; R-PTP-zeta; RPTPz; Rptpbeta
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ptprz1em1flox/Cya mice (Catalog S-CKO-04616) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000090568
NCBI RefSeq
NM_001081306
Target Region
Exon 3~4
Size of Effective Region
~3.3 kb
Detailed Document
Overview of Gene Research
Ptprz1, or protein tyrosine phosphatase receptor type Z, is involved in multiple cellular functions. It has been linked to pathways related to cell proliferation, adhesion, and migration. In the context of cancer, it is associated with signaling that impacts tumor growth, making it of great biological importance. Genetic models, such as gene knockout (KO) and conditional knockout (CKO) mouse models, are valuable for studying its functions [8].
In glioblastoma, Ptprz1 has been a key focus. Tumour-associated macrophages secrete pleiotrophin (PTN) which binds to Ptprz1 on glioblastoma stem cells, promoting tumour growth. Disrupting Ptprz1 abrogates glioblastoma stem cell maintenance and tumorigenic potential, and blocking the PTN-Ptprz1 signalling suppresses tumour growth and prolongs animal survival [1]. Anti-Ptprz1 RNA CAR T cells show antigen-specific and bystander antitumor activity in glioblastoma, validating Ptprz1 as a glioblastoma target [2]. The PTPRZ1-MET fusion is associated with glioma progression, and targeting this fusion with a MET inhibitor is being investigated in a clinical trial [3]. Ptprz1-MET fusion in glioma stem-like cells modulates tumor-associated macrophage polarization, revealing a new mechanism in glioblastoma pathogenesis [4]. A novel PTPRZ1-ETV1 fusion has been detected in gliomas, potentially presenting a new therapeutic target [5]. Blocking the PTN-Ptprz1 signaling axis remodels the tumor microenvironment in glioblastoma, offering a new treatment strategy [6]. In clear cell renal cell carcinoma (ccRCC), Ptprz1 dephosphorylates and stabilizes RNF26, reducing the efficacy of tyrosine kinase inhibitors (TKIs) and PD-1 blockade. Inhibiting Ptprz1 enhances the sensitivity of ccRCC to these treatments [7].
In conclusion, Ptprz1 is significantly involved in tumor-related biological processes, especially in glioblastoma and ccRCC. Studies using KO or CKO mouse models, though not always explicitly detailed in the references, would likely further clarify its role. The research on Ptprz1 provides potential therapeutic targets for treating these cancers, highlighting its importance in understanding disease mechanisms and developing treatment strategies.
References:
1. Shi, Yu, Ping, Yi-Fang, Zhou, Wenchao, Bao, Shideng, Bian, Xiu-Wu. 2017. Tumour-associated macrophages secrete pleiotrophin to promote PTPRZ1 signalling in glioblastoma stem cells for tumour growth. In Nature communications, 8, 15080. doi:10.1038/ncomms15080. https://pubmed.ncbi.nlm.nih.gov/28569747/
2. Martinez Bedoya, Darel, Marinari, Eliana, Davanture, Suzel, Dutoit, Valérie, Migliorini, Denis. . PTPRZ1-Targeting RNA CAR T Cells Exert Antigen-Specific and Bystander Antitumor Activity in Glioblastoma. In Cancer immunology research, 12, 1718-1735. doi:10.1158/2326-6066.CIR-23-1094. https://pubmed.ncbi.nlm.nih.gov/39269445/
3. Bao, Zhaoshi, Li, Shouwei, Wang, Liang, Qiu, Xiaoguang, Jiang, Tao. 2023. PTPRZ1-METFUsion GENe (ZM-FUGEN) trial: study protocol for a multicentric, randomized, open-label phase II/III trial. In Chinese neurosurgical journal, 9, 21. doi:10.1186/s41016-023-00329-0. https://pubmed.ncbi.nlm.nih.gov/37443050/
4. Wang, Yuxin, Suo, Jinghao, Wang, Zhixing, Liu, Yanwei, Peng, Xiaozhong. 2024. The PTPRZ1-MET/STAT3/ISG20 axis in glioma stem-like cells modulates tumor-associated macrophage polarization. In Cellular signalling, 120, 111191. doi:10.1016/j.cellsig.2024.111191. https://pubmed.ncbi.nlm.nih.gov/38685521/
5. Matjašič, Alenka, Zupan, Andrej, Boštjančič, Emanuela, Popović, Mara, Kolenc, Danijela. 2019. A novel PTPRZ1-ETV1 fusion in gliomas. In Brain pathology (Zurich, Switzerland), 30, 226-234. doi:10.1111/bpa.12776. https://pubmed.ncbi.nlm.nih.gov/31381204/
6. Yang, Meng, Wang, Bingyi, Yin, Ying, Yin, Tieying, Wang, Yazhou. 2022. PTN-PTPRZ1 signaling axis blocking mediates tumor microenvironment remodeling for enhanced glioblastoma treatment. In Journal of controlled release : official journal of the Controlled Release Society, 353, 63-76. doi:10.1016/j.jconrel.2022.11.025. https://pubmed.ncbi.nlm.nih.gov/36402232/
7. Ma, Yongkang, Li, Wei, Liu, Xinlin, Liu, Wentao, Chen, Xiaobing. 2024. PTPRZ1 dephosphorylates and stabilizes RNF26 to reduce the efficacy of TKIs and PD-1 blockade in ccRCC. In Oncogene, 43, 3633-3644. doi:10.1038/s41388-024-03198-8. https://pubmed.ncbi.nlm.nih.gov/39443724/
8. Xia, Zhenkun, Ouyang, Dengjie, Li, Qianying, Yi, Wenjun, Zhou, Enxiang. 2019. The Expression, Functions, Interactions and Prognostic Values of PTPRZ1: A Review and Bioinformatic Analysis. In Journal of Cancer, 10, 1663-1674. doi:10.7150/jca.28231. https://pubmed.ncbi.nlm.nih.gov/31205522/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen