C57BL/6NCya-Ndc80em1flox/Cya
Common Name:
Ndc80-flox
Product ID:
S-CKO-13473
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Ndc80-flox
Strain ID
CKOCMP-67052-Ndc80-B6N-VA
Gene Name
Product ID
S-CKO-13473
Gene Alias
2610020P18Rik; HEC; HEC1; Kntc2
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
17
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Ndc80em1flox/Cya mice (Catalog S-CKO-13473) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000024851
NCBI RefSeq
NM_023294
Target Region
Exon 4~7
Size of Effective Region
~4.4 kb
Detailed Document
Overview of Gene Research
Ndc80, also known as Hec1 (highly expressed in cancer 1), is a key component of the Ndc80 complex. This complex is a core part of the kinetochore-microtubule attachment machinery in eukaryotic cells, playing a crucial role in successful mitotic cell division by mediating the attachments between chromosomes and spindle microtubules [1,3,4,6,7,8,9,10]. These attachments need to be stable yet regulated for timely and error-free mitotic progression. The Ndc80 complex is also involved in processes like chromosome congress and spindle checkpoint signaling [6,9].
In triple-negative breast cancer (TNBC), Ndc80 has been found to be a cisplatin-resistant gene. Its expression is upregulated in cisplatin-resistant cells and TNBC tissues, and overexpression promotes cell viability, proliferation, and cisplatin resistance [2]. In glioma, Hec1 (a component of the Ndc80 complex) overexpression is observed, indicating poor prognosis. It promotes cell proliferation, the formation of an immunosuppressive tumor microenvironment, and the transformation of M1 to M2 macrophages [5].
In conclusion, Ndc80 is essential for kinetochore-microtubule attachments and normal mitotic cell division. Its dysregulation is associated with diseases like TNBC and glioma. Research on Ndc80 helps in understanding the mechanisms of drug resistance and tumorigenesis, potentially providing new therapeutic targets for these diseases.
References：
1. Wimbish, Robert T, DeLuca, Jennifer G. 2020. Hec1/Ndc80 Tail Domain Function at the Kinetochore-Microtubule Interface. In Frontiers in cell and developmental biology, 8, 43. doi:10.3389/fcell.2020.00043. https://pubmed.ncbi.nlm.nih.gov/32161753/
2. Li, Jing, Xu, Xiaoqin, Peng, Xiting. 2022. NDC80 Enhances Cisplatin-resistance in Triple-negative Breast Cancer. In Archives of medical research, 53, 378-387. doi:10.1016/j.arcmed.2022.03.003. https://pubmed.ncbi.nlm.nih.gov/35346500/
3. Chen, Jingxun, Ünal, Elçin. 2021. Meiotic regulation of the Ndc80 complex composition and function. In Current genetics, 67, 511-518. doi:10.1007/s00294-021-01174-3. https://pubmed.ncbi.nlm.nih.gov/33745061/
4. Ustinov, N B, Korshunova, A V, Gudimchuk, N B. . Protein Complex NDC80: Properties, Functions, and Possible Role in Pathophysiology of Cell Division. In Biochemistry. Biokhimiia, 85, 448-462. doi:10.1134/S0006297920040057. https://pubmed.ncbi.nlm.nih.gov/32569552/
5. Ye, Weijie, Liang, Xisong, Chen, Ge, Cheng, Quan, Chen, Xiaoping. . NDC80/HEC1 promotes macrophage polarization and predicts glioma prognosis via single-cell RNA-seq and in vitro experiment. In CNS neuroscience & therapeutics, 30, e14850. doi:10.1111/cns.14850. https://pubmed.ncbi.nlm.nih.gov/39021287/
6. Tooley, John, Stukenberg, P Todd. . The Ndc80 complex: integrating the kinetochore's many movements. In Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 19, 377-91. doi:10.1007/s10577-010-9180-5. https://pubmed.ncbi.nlm.nih.gov/21311965/
7. Polley, Soumitra, Müschenborn, Helen, Terbeck, Melina, Volkov, Vladimir A, Huis In 't Veld, Pim J. 2023. Stable kinetochore-microtubule attachment requires loop-dependent Ndc80-Ndc80 binding. In The EMBO journal, 42, e112504. doi:10.15252/embj.2022112504. https://pubmed.ncbi.nlm.nih.gov/37203876/
8. Rahi, Amit, Chakraborty, Manas, Agarwal, Shivangi, McKenney, Richard J, Varma, Dileep. 2023. The Ndc80-Cdt1-Ska1 complex is a central processive kinetochore-microtubule coupling unit. In The Journal of cell biology, 222, . doi:10.1083/jcb.202208018. https://pubmed.ncbi.nlm.nih.gov/37265445/
9. Ciferri, Claudio, Musacchio, Andrea, Petrovic, Arsen. 2007. The Ndc80 complex: hub of kinetochore activity. In FEBS letters, 581, 2862-9. doi:. https://pubmed.ncbi.nlm.nih.gov/17521635/
10. Muir, Kyle W, Batters, Christopher, Dendooven, Tom, Burt, Alister, Barford, David. 2023. Structural mechanism of outer kinetochore Dam1-Ndc80 complex assembly on microtubules. In Science (New York, N.Y.), 382, 1184-1190. doi:10.1126/science.adj8736. https://pubmed.ncbi.nlm.nih.gov/38060647/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen