C57BL/6JCya-1700102P08Rikem1/Cya
Common Name:
1700102P08Rik-KO
Product ID:
S-KO-18270
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
1700102P08Rik-KO
Strain ID
KOCMP-112418-1700102P08Rik-B6J-VA
Gene Name
Product ID
S-KO-18270
Gene Alias
-
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-1700102P08Rikem1/Cya mice (Catalog S-KO-18270) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000035234
NCBI RefSeq
NM_053216
Target Region
Exon 3
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
1700102P08Rik, also named male pachynema-specific (MAPS) protein, is a testis-specific gene conserved in human and mouse. It is essential for spermatogenesis, especially for pachynema progression in spermatocytes [1,3]. It is involved in the process of male germ cell development and its proper function is crucial for male fertility. Gene knockout mouse models have been key in studying its function [1-3].
Knockout mouse models generated by CRISPR-Cas9 showed that 1700102P08Rik knockout male mice were infertile with smaller testis and epididymis, while female knockout mice had normal fertility. Spermatogenesis in male knockout mice was arrested at the spermatocyte stage, with no sperm in the epididymis, and deletion of this gene caused apoptosis in the testis. It was also found to be down-regulated in spermatocyte arrest in men [1]. Additionally, FuncProFinder, a machine-learning-based tool, predicted and validated 1700102P08Rik as a meiosis-essential gene based on spermatogenesis proteome dynamics [2]. Mice lacking the protein encoded by 1700102P08Rik (Maps-/-) suffered from pachytene arrest and spermatocyte death, leading to male infertility. Pubertal Maps-/-spermatocytes had defects in DNA double-strand break repair, crossover formation, and XY body formation, while adult Maps-/-spermatocytes had partially defective crossover and failed pachytene stage progression [3].
In conclusion, 1700102P08Rik is essential for spermatogenesis and male fertility. The gene knockout mouse models have revealed its crucial role in male-specific reproductive processes, providing valuable insights into the mechanisms of male infertility [1-3].
References:
1. Wu, Xiao-Long, Yun, Da-Min, Gao, Sheng, Wang, Gui-Shuan, Sun, Fei. 2020. The testis-specific gene 1700102P08Rik is essential for male fertility. In Molecular reproduction and development, 87, 231-240. doi:10.1002/mrd.23314. https://pubmed.ncbi.nlm.nih.gov/31930642/
2. Fang, Kailun, Li, Qidan, Wei, Yu, Liu, Siqi, Chen, Charlie Degui. 2021. Prediction and Validation of Mouse Meiosis-Essential Genes Based on Spermatogenesis Proteome Dynamics. In Molecular & cellular proteomics : MCP, 20, 100014. doi:10.1074/mcp.RA120.002081. https://pubmed.ncbi.nlm.nih.gov/33257503/
3. Li, Miao, Zheng, Jiahuan, Li, Gaopeng, Wang, Hengbin, Liu, Kui. . The male germline-specific protein MAPS is indispensable for pachynema progression and fertility. In Proceedings of the National Academy of Sciences of the United States of America, 118, . doi:10.1073/pnas.2025421118. https://pubmed.ncbi.nlm.nih.gov/33602822/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen