Regular transgenesis is a widely used method for generating rodent models with strong transgene expression. Through pronuclear injection, it enables in vivo studies of gene overexpression and regulatory elements.

In regular transgenesis, linearized DNA constructs are microinjected into the pronucleus of fertilized mouse or rat embryos. These constructs integrate randomly into the host genome, typically in multiple copies, and result in hemizygous expression of the transgene. The founders are screened for integration and expression, then bred for colony expansion or experimental use.

• Random genomic integration with multi-copy insertion per site.
• Variable expression patterns among founders.
• Often strong expression in early generations, with potential for position-effect variegation due to integration site randomness.

• Gene overexpression or dominant-negative constructs.
• Tissue-specific expression using conditional promoters.
• Large DNA insertions via BAC vectors.

BAC transgenesis involves injecting bacterial artificial chromosome constructs into fertilized zygotes. These BACs carry large genomic regions, including promoters, exons, introns, and enhancers, enabling expression patterns that closely mimic native gene regulation. Because the integration is random and multicopy, BAC models are typically screened for expression and copy number.

Technical Distinction: Compared to knockin strategies using homologous recombination or gene editing, BAC transgenesis allows the introduction of entire genes without modifying endogenous loci, making it ideal for exploratory or overexpression studies.

• Studying intact human gene expression.
• Investigating long-range regulatory interactions.
• Analyzing noncoding RNAs in their native context.
• Delivering multigene constructs that exceed the capacity of conventional vectors.

Our PiggyBac transgenesis platform enables efficient generation of stable, single-copy transgenic rodent models with consistent gene expression. It offers a reliable and cost-effective alternative for studying gene function and overexpression in vivo.

PiggyBac transgenesis introduces a defined DNA fragment into the genome via pronuclear injection. Unlike traditional methods, it integrates a single copy of the transgene without vector backbone or sequence truncation, resulting in more stable and predictable expression patterns.

For researchers requiring large genomic regions with regulatory elements, our PiggyBac-on-BAC method combines the capacity of BACs (approximately 100 to 300 kb) with the single-copy fidelity of PiggyBac. This enables faithful gene expression patterns while avoiding multi-copy integration artifacts commonly seen in conventional transgenics.

This platform is especially suited for in vivo studies requiring stable, high-fidelity expression of transgenes. Its ability to support single-copy integration with minimal disruption makes it ideal for:

• Gene function analysis and regulatory element studies.
• Therapeutic gene delivery validation.
• Modeling human diseases, including those involving large or complex sequences like lncRNAs.