This model is Il10 gene knockout mice, where the Il10 gene homologous to the human IL10 gene has been knocked out in BALB/cAnCya mice. Homozygous BALB/c-Il10 KO mice are viable and fertile. At 8 to 9 weeks of age, BALB/c-Il10 KO mice spontaneously develop colitis symptoms, characterized by weight loss, reduced survival rates, elevated levels of inflammatory factors, and abnormalities in intestinal inflammation and phenotype. BALB/c-Il10 KO mice can be used for research related to Crohn’s disease (CD), colitis, other inflammatory bowel diseases (IBD), cancer, congenital and adaptive immune disorders, as well as various inflammation or autoimmune conditions. It should be noted that due to individual variability and environmental factors, the disease presentation of this model may vary. The data in this manual are based on internal facility observations and are provided for reference. Actual disease presentation may vary; please use the mice according to your specific experimental conditions for best results.

This strain is an Il10 gene knockout (Il10 KO) mouse, in which the Il10 gene homologous to the human IL10 gene has been knocked out in mice using gene editing technology. Homozygous Il10-KO mice do not produce the anti-inflammatory cytokine IL-10 and can survive and reproduce under specific pathogen-free (SPF) conditions. This strain is maintained in a high-standard SPF environment in Cyagen facilities, and its phenotype differs from that of Il10 knockout mice raised in conventional mouse facilities as reported in the literature. For example, the development of colitis in this model may be slow or non-spontaneous, but the introduction of microbes or fecal microbiota may cause rapid development of colitis in adult animals. Homozygous Il10 KO mice are viable and fertile.

Il10 is located on chromosome 1 of mice. Nuclease Technology was used to design sgRNA; Il10 knockout mice were obtained by applying high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.

Il10 is located on chromosome 1 of mice. SgRNA and ssDNA will be designed using Nuclease Technology; Il10 conditional knockout mice will be obtained by high-throughput electroporation of fertilized eggs. After sexual maturity, sperm will be collected for cryopreservation.

Il10ra is located on chromosome 9 of mice. Nuclease Technology will be used to design sgRNA; Il10ra knockout mice will be obtained by applying high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.

Il10ra is located on chromosome 9 of mice. Nuclease Technology will be used to design sgRNA; Il10ra knockout mice will be obtained by applying high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.

Il10rb is located on chromosome 16 of mice. Nuclease Technology will be used to design sgRNA; Il10rb knockout mice will be obtained by applying high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.

Il10ra is located on chromosome 9 of mice. SgRNA and ssDNA will be designed using Nuclease Technology; Il10ra conditional knockout mice will be obtained by high-throughput electroporation of fertilized eggs. After sexual maturity, sperm will be collected for cryopreservation.

Il10rb is located on chromosome 16 of mice. SgRNA and ssDNA were designed using Nuclease Technology; Il10rb conditional knockout mice were obtained by high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.

Rhbdf2 is located on chromosome 11 of mice. SgRNA and ssDNA were designed using Nuclease Technology; Rhbdf2 conditional knockout mice were obtained by high-throughput electroporation of fertilized eggs. After sexual maturity, sperm were collected for cryopreservation.