Il10 KO Mouse
Product Name
Il10 KO Mouse
Product ID
C001230
Strain Name
C57BL/6NCya-Il10em1/Cya
Backgroud
C57BL/6NCya
Status
When using this mouse strain in a publication, please cite “Il10 KO Mouse (Catalog C001230) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Gene Name
Il10
Gene Alias
CSIF, If2a, Il-10
NCBI ID
Chromosome
Chr 1 (Mouse)
MGI ID
Datasheet
Strain Description
Interleukin 10 (IL-10) is a cytokine with a wide range of biological activities, secreted by antigen-presenting cells such as activated T cells, monocytes, B cells, and macrophages. IL-10 plays a pleiotropic role in immunoregulation and inflammation, downregulating the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages, and enhancing the survival, proliferation, and antibody production of B cells. IL-10 can block NF-kB activity and participate in the regulation of the JAK-STAT signaling pathway. In addition, IL-10 can also activate PI3K and its downstream substrates p70S6K and Akt/PKB. Overexpression of IL-10 (such as in systemic lupus erythematosus, tuberculosis) and IL-10 deficiency (such as in inflammatory bowel disease, psoriasis, asthma, rheumatoid arthritis) have different pathophysiological significance. Therefore, neutralizing cytokines may be an effective method for treating the former group of diseases, while the application of IL-10 itself may help treat the latter group of diseases.
This strain is an Il10 gene knockout (Il10 KO) mouse, in which the Il10 gene homologous to the human IL10 gene has been knocked out in mice using gene editing technology. Homozygous Il10-KO mice do not produce the anti-inflammatory cytokine IL-10 and can survive and reproduce under specific pathogen-free (SPF) conditions. This strain is maintained in a high-standard SPF environment in Cyagen facilities, and its phenotype differs from that of Il10 knockout mice raised in conventional mouse facilities as reported in the literature. For example, the development of colitis in this model may be slow or non-spontaneous, but the introduction of microbes or fecal microbiota may cause rapid development of colitis in adult animals. Homozygous Il10 KO mice are viable and fertile.
Strain Strategy
The Il10 gene is located on mouse chromosome 1, and Exon 1 to 5 of this gene was knocked out using gene editing techniques.
Figure 1. Diagram of the gene editing strategy for the generation of Il10 KO mice.
Application Area
Il10 KO mice can be used for research in areas including inflammatory bowel disease (IBD), such as Crohn’s disease (CD) and colitis, cancer, congenital and adaptive immune diseases, and many other inflammation or autoimmune research fields.
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