C57BL/6JCya-Dmrta1em1/Cya
Common Name
Dmrta1-KO
Product ID
S-KO-07374
Backgroud
C57BL/6JCya
Strain ID
KOCMP-242523-Dmrta1-B6J-VA
Status
When using this mouse strain in a publication, please cite “Dmrta1-KO Mouse (Catalog S-KO-07374) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Dmrta1-KO
Strain ID
KOCMP-242523-Dmrta1-B6J-VA
Gene Name
Product ID
S-KO-07374
Gene Alias
Dmrt4
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 4 (Mouse)
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Transcript ID
ENSMUST00000052478
NCBI Transcript ID
NM_175647
Target Region
Exon 1~2
Size of Effective Region
~7.1 kb
Overview of Gene Research
Dmrta1, or doublesex and mab-3-related transcription factor-like family A1, is a transcription factor involved in various biological processes. It plays a role in neural development, such as in the mammalian telencephalon where it may be regulated by Pax6 and is involved in the balance of cell proliferation and neuronal differentiation by regulating proneural gene expression [2]. It is also associated with otic development as Sf3b4 knockdown in Xenopus embryos led to reduced dmrta1 expression and affected otic development [5].
In esophageal squamous cell carcinoma (ESCC), knockdown of Dmrta1 decreased the proliferation rate of esophageal squamous carcinoma cells, increased cisplatin sensitivity, and decreased immune escape. Mechanistically, Dmrta1 forms a positive-feedback loop with SOX2 by binding to the promoter of SOX2, promoting ESCC progression and chemoresistance [1]. In dental fear and anxiety, a genome-wide association study found a significant association between a genetic locus near Dmrta1 and dental fear and anxiety sub-scales [3]. Also, in pan-cancer in silico analysis, deletion of Dmrta1 in the chr9p22.1-21.3 locus was associated with poor survival and increased hazard ratio in sarcoma datasets [4].
In conclusion, Dmrta1 is crucial in neural and otic development. Its role in disease, especially in ESCC where it impacts chemotherapy resistance and immune escape, highlights its potential as a therapeutic target. The association with dental fear and anxiety and cancer survival further emphasizes its importance in understanding the genetic basis of these conditions.
References:
1. Zhang, Rui, Zhou, Peng, Ou, Xia, Xi, Mian, Qing, Chen. 2023. The DMRTA1-SOX2 positive feedback loop promotes progression and chemotherapy resistance of esophageal squamous cell carcinoma. In Oncology research, 31, 887-897. doi:10.32604/or.2023.030184. https://pubmed.ncbi.nlm.nih.gov/37744275/
2. Kikkawa, Takako, Obayashi, Takeshi, Takahashi, Masanori, Numayama-Tsuruta, Keiko, Osumi, Noriko. 2013. Dmrta1 regulates proneural gene expression downstream of Pax6 in the mammalian telencephalon. In Genes to cells : devoted to molecular & cellular mechanisms, 18, 636-49. doi:10.1111/gtc.12061. https://pubmed.ncbi.nlm.nih.gov/23679989/
3. Zhou, Y, McNeil, D W, Haworth, S, Marazita, M L, Shaffer, J R. 2022. Genome-wide Scan of Dental Fear and Anxiety Nominates Novel Genes. In Journal of dental research, 101, 1526-1536. doi:10.1177/00220345221105226. https://pubmed.ncbi.nlm.nih.gov/35771046/
4. Gonçalves, Paola G, Reis, Rui M, Bidinotto, Lucas T. . Significance of Chr9p22.1-p21.3 Deletion in Cancer Development: A Pan-cancer In Silico Analysis. In Anticancer research, 42, 5291-5304. doi:10.21873/anticanres.16036. https://pubmed.ncbi.nlm.nih.gov/36288884/
5. Maharana, Santosh Kumar, Saint-Jeannet, Jean-Pierre. 2021. Molecular mechanisms of hearing loss in Nager syndrome. In Developmental biology, 476, 200-208. doi:10.1016/j.ydbio.2021.04.002. https://pubmed.ncbi.nlm.nih.gov/33864777/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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