C57BL/6JCya-Lhfpl5em1/Cya
Common Name
Lhfpl5-KO
Product ID
S-KO-09428
Backgroud
C57BL/6JCya
Strain ID
KOCMP-328789-Lhfpl5-B6J-VA
Status
When using this mouse strain in a publication, please cite “Lhfpl5-KO Mouse (Catalog S-KO-09428) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Lhfpl5-KO
Strain ID
KOCMP-328789-Lhfpl5-B6J-VA
Gene Name
Product ID
S-KO-09428
Gene Alias
Tmhs, hscy, 9330179O15Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 17 (Mouse)
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Transcript ID
ENSMUST00000080780
NCBI Transcript ID
NM_026571
Target Region
Exon 1~3
Size of Effective Region
~8.4 kb
Overview of Gene Research
Lhfpl5, lipoma HMGIC fusion partner-like 5, is a crucial transmembrane protein involved in the mechano-electrical transduction (MET) process in cochlear hair cells. It plays a vital role in our senses of hearing and balance by enabling the conversion of mechanical stimuli into electrical signals. Lhfpl5 is thought to form a multi-protein complex with other MET channel proteins such as PCDH15, TMIE, and TMC1 [2].
In Lhfpl5 -/- knockout mice, compared with Lhfpl5+/- heterozygotes, the working range of MET channel activation in outer hair cells increased, and the single-channel gating force decreased, indicating reduced sensitivity. The gating stiffness, a key factor in channel activation, was virtually abolished in Lhfpl5 -/-, suggesting LHFPL5 is a principal component of the gating spring that transmits tip-link tension to the MET channel [1]. Also, disruption of the interaction between LHFPL5 and TMC1, like in the Tmc1 p.D569N mutation, which reduced LHFPL5 immunolabeling in stereocilia, led to a doubling of the MET working range [1].
In conclusion, Lhfpl5 is essential for establishing the maximal force sensitivity of the MET channel in cochlear hair cells. Its role in directly coupling the tip link to the MET channel is demonstrated through gene knockout mouse models. Mutations in Lhfpl5 can cause non-syndromic autosomal recessive hearing loss, highlighting its significance in understanding the genetic basis of hearing disorders [1,3].
References:
1. Beurg, Maryline, Schwalbach, Evan Travis, Fettiplace, Robert. 2024. LHFPL5 is a key element in force transmission from the tip link to the hair cell mechanotransducer channel. In Proceedings of the National Academy of Sciences of the United States of America, 121, e2318270121. doi:10.1073/pnas.2318270121. https://pubmed.ncbi.nlm.nih.gov/38194445/
2. Soler, David C, Ballesteros, Angela, Sloan, Andrew E, McCormick, Thomas S, Stepanyan, Ruben. 2023. Multiple plasma membrane reporters discern LHFPL5 region that blocks trafficking to the plasma membrane. In Scientific reports, 13, 2528. doi:10.1038/s41598-023-28045-w. https://pubmed.ncbi.nlm.nih.gov/36781873/
3. Al-Amri, Ahmed H, Al Saegh, Abeer, Al-Mamari, Watfa, Clapcote, Steven J, Ali, Manir. 2018. LHFPL5 mutation: A rare cause of non-syndromic autosomal recessive hearing loss. In European journal of medical genetics, 62, 103592. doi:10.1016/j.ejmg.2018.11.026. https://pubmed.ncbi.nlm.nih.gov/30476627/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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