C57BL/6JCya-Pwp1em1/Cya
Common Name
Pwp1-KO
Product ID
S-KO-17498
Backgroud
C57BL/6JCya
Strain ID
KOCMP-103136-Pwp1-B6J-VA
Status
When using this mouse strain in a publication, please cite “Pwp1-KO Mouse (Catalog S-KO-17498) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Pwp1-KO
Strain ID
KOCMP-103136-Pwp1-B6J-VA
Gene Name
Product ID
S-KO-17498
Gene Alias
2310058A11Rik, 2610205J09Rik
Background
C57BL/6JCya
Gene Full Name
PWP1 homolog, endonuclein
Modification
Conventional knockout
NCBI ID
Phenotype
Chromosome
Chr 10 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000001836
NCBI Transcript ID
NM_133993
Target Region
Exon 2~3
Size of Effective Region
~1.6 kb
Overview of Gene Research
Pwp1, also known as Periodic tryptophan protein 1 homolog or endonuclein, is a chromatin - binding protein with diverse functions. It is involved in multiple essential biological processes, such as telomere length regulation, ribosome biogenesis, and the regulation of signaling pathways like Wnt and Hippo. It also plays a role in cell differentiation, development, and nutrient - dependent growth control [2,3,4,5,6].
Homozygous Pwp1 - knockout mice and embryonic stem cells (ESCs) have never been obtained, indicating its essentiality for mouse development and ESC survival. Heterozygous Pwp1 - knockout mice had shorter telomeres and decreased reproductive capacity. Pwp1 depletion induced rapid telomere shortening, reduced shelterin complex, and increased DNA damage in telomeric regions [2]. In mouse embryonic stem cells (mESCs), knockdown of Pwp1 impairs their differentiation potential both in vitro and in vivo [5]. In mouse preimplantation embryos, Pwp1 inhibition reduces developmental potential and causes irregular localization of YAP1, abnormal regulation of inner cell mass and trophectoderm markers, and upregulation of ZGA gene expression [8]. In cancer, PWP1 is overexpressed in hepatocellular carcinoma, lung cancer, and gastric cancer, promoting cancer cell proliferation, invasion, and metastasis. For example, siRNA - mediated PWP1 silencing significantly suppressed HCC cell proliferation in vitro [1,3,7].
In conclusion, Pwp1 is crucial for various biological functions including telomere maintenance, cell differentiation, and ribosome biogenesis. The study of Pwp1 knockout models has revealed its significant roles in development and disease, especially in cancer. These findings suggest that Pwp1 could be a potential therapeutic target for treating certain cancers [1,2,3,5,7,8].
References：
1. Huang, Rong, Xu, Fu, Su, Luying, Su, Liya, Song, Wei. 2024. PWP1 is overexpressed in hepatocellular carcinoma and facilitates liver cancer cell proliferation. In Heliyon, 10, e32409. doi:10.1016/j.heliyon.2024.e32409. https://pubmed.ncbi.nlm.nih.gov/38933950/
2. Yu, Yangyang, Jia, Wenwen, Lyu, Yao, Zhu, Jiyue, Kang, Jiuhong. 2019. Pwp1 regulates telomere length by stabilizing shelterin complex and maintaining histone H4K20 trimethylation. In Cell discovery, 5, 47. doi:10.1038/s41421-019-0116-8. https://pubmed.ncbi.nlm.nih.gov/31754456/
3. Wei, Lai, Li, Pengcheng, Luo, Yuan, Liu, Shuli, Wang, Enhua. 2020. PWP1 Promotes the Malignant Phenotypes of Lung Cancer Cells by Interacting with DVL2 and Merlin. In OncoTargets and therapy, 13, 10025-10037. doi:10.2147/OTT.S263815. https://pubmed.ncbi.nlm.nih.gov/33116587/
4. Liu, Ying, Cerejeira Matos, Rita, Heino, Tapio I, Hietakangas, Ville. 2018. PWP1 promotes nutrient-responsive expression of 5S ribosomal RNA. In Biology open, 7, . doi:10.1242/bio.037911. https://pubmed.ncbi.nlm.nih.gov/30361412/
5. Shen, Junwei, Jia, Wenwen, Yu, Yangyang, Jiang, Cizhong, Kang, Jiuhong. . Pwp1 is required for the differentiation potential of mouse embryonic stem cells through regulating Stat3 signaling. In Stem cells (Dayton, Ohio), 33, 661-73. doi:10.1002/stem.1876. https://pubmed.ncbi.nlm.nih.gov/25335925/
6. Liu, Ying, Mattila, Jaakko, Ventelä, Sami, Westermarck, Jukka, Hietakangas, Ville. . PWP1 Mediates Nutrient-Dependent Growth Control through Nucleolar Regulation of Ribosomal Gene Expression. In Developmental cell, 43, 240-252.e5. doi:10.1016/j.devcel.2017.09.022. https://pubmed.ncbi.nlm.nih.gov/29065309/
7. Jiang, Mingrui, Wang, Sen, Ji, Jin, Wang, Wei, Wang, Daorong. 2024. PWP1 transcriptionally regulates p53, modulating apoptosis and cell cycle to promote gastric cancer progression. In Apoptosis : an international journal on programmed cell death, 30, 693-709. doi:10.1007/s10495-024-02049-x. https://pubmed.ncbi.nlm.nih.gov/39720977/
8. Yamamoto, Takuto, Takasu, Atsushi, Isumi, Yasuhiro, Minami, Naojiro, Ikeda, Shuntaro. 2025. Pwp1 inhibition impairs the development and early lineage commitment of mouse preimplantation embryos. In The Journal of reproduction and development, , . doi:10.1262/jrd.2024-111. https://pubmed.ncbi.nlm.nih.gov/40335308/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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