C57BL/6JCya-Dlk1em1/Cya
Common Name
Dlk1-KO
Product ID
S-KO-17887
Backgroud
C57BL/6JCya
Strain ID
KOCMP-13386-Dlk1-B6J-VA
Status
When using this mouse strain in a publication, please cite “Dlk1-KO Mouse (Catalog S-KO-17887) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Dlk1-KO
Strain ID
KOCMP-13386-Dlk1-B6J-VA
Gene Name
Product ID
S-KO-17887
Gene Alias
FA1, ZOG, pG2, DlkI, Peg9, SCP1, DLK-1, Ly107, pref-1
Background
C57BL/6JCya
Gene Full Name
delta like non-canonical Notch ligand 1
Modification
Conventional knockout
NCBI ID
Phenotype
Chromosome
Chr 12 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000109844
NCBI Transcript ID
NM_010052
Target Region
Exon 1~5
Size of Effective Region
~7.8 kb
Overview of Gene Research
DLK1, also known as delta-like canonical notch ligand 1, is a maternally imprinted, paternally expressed gene encoding a transmembrane protein. It is a non-canonical NOTCH ligand and plays significant roles in adipose tissue homeostasis, neurogenesis, stem cell pool regulation, and tissue differentiation [1,3]. It is located in the imprinted chromosome 14q32 region, and its deregulation is associated with developmental disorders like Temple syndrome [1,4].
In terms of disease-related findings, paternally inherited genetic defects of DLK1 were identified in families with nonsyndromic central precocious puberty (CPP) and a metabolic phenotype, suggesting its role in modulating the hypothalamic-pituitary-gonadal axis [1]. In neuroblastoma, high expression of DLK1 directly correlates with a super-enhancer, and its silencing leads to increased cellular differentiation. An antibody-drug conjugate targeting DLK1 shows potent cytotoxicity in xenograft models, indicating its potential as an immunotherapeutic target [2]. In adrenocortical carcinoma, DLK1 is a prognostic marker, and its cleavable ectodomain in serum can aid in diagnosis [5]. Also, in hepatocellular carcinoma, DLK1-directed chimeric antigen receptor T-cell therapy can specifically eliminate DLK1-positive cancer cells [6].
In conclusion, DLK1 is crucial for multiple biological processes and is associated with various diseases. Studies on genetic defects and expression patterns in different disease models, such as in CPP, neuroblastoma, adrenocortical carcinoma, and hepatocellular carcinoma, have revealed its significance in disease development and its potential as a therapeutic target. Understanding DLK1 may provide new strategies for treating these diseases.
References：
1. Macedo, Delanie B, Kaiser, Ursula B. 2020. DLK1, Notch Signaling and the Timing of Puberty. In Seminars in reproductive medicine, 37, 174-181. doi:10.1055/s-0039-3400963. https://pubmed.ncbi.nlm.nih.gov/31972862/
2. Hamilton, Amber K, Radaoui, Alexander B, Tsang, Matthew, Maris, John M, Diskin, Sharon J. 2024. A proteogenomic surfaceome study identifies DLK1 as an immunotherapeutic target in neuroblastoma. In Cancer cell, 42, 1970-1982.e7. doi:10.1016/j.ccell.2024.10.003. https://pubmed.ncbi.nlm.nih.gov/39454577/
3. Grassi, Elisa Stellaria, Pietras, Alexander. 2021. Emerging Roles of DLK1 in the Stem Cell Niche and Cancer Stemness. In The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 70, 17-28. doi:10.1369/00221554211048951. https://pubmed.ncbi.nlm.nih.gov/34606325/
4. Enterina, Jhon R, Enfield, Katey S S, Anderson, Christine, Ng, Kevin W, Lam, Wan L. 2017. DLK1-DIO3 imprinted locus deregulation in development, respiratory disease, and cancer. In Expert review of respiratory medicine, 11, 749-761. doi:10.1080/17476348.2017.1355241. https://pubmed.ncbi.nlm.nih.gov/28715922/
5. Mariniello, Katia, Pittaway, James F H, Altieri, Barbara, Fassnacht, Martin, Guasti, Leonardo. 2024. Dlk1 is a novel adrenocortical stem/progenitor cell marker that predicts malignancy in adrenocortical carcinoma. In bioRxiv : the preprint server for biology, , . doi:10.1101/2024.08.22.609117. https://pubmed.ncbi.nlm.nih.gov/39229217/
6. Zhai, Yangyang, He, Kunyan, Huang, Liyu, Yuan, Guandou, Han, Ze-Guang. 2022. DLK1-directed chimeric antigen receptor T-cell therapy for hepatocellular carcinoma. In Liver international : official journal of the International Association for the Study of the Liver, 42, 2524-2537. doi:10.1111/liv.15411. https://pubmed.ncbi.nlm.nih.gov/36002393/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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