C57BL/6JCya-Fmnl1em1/Cya
Common Name
Fmnl1-KO
Product ID
S-KO-20013
Backgroud
C57BL/6JCya
Strain ID
KOCMP-57778-Fmnl1-B6J-VB
Status
When using this mouse strain in a publication, please cite “Fmnl1-KO Mouse (Catalog S-KO-20013) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Fmnl1-KO
Strain ID
KOCMP-57778-Fmnl1-B6J-VB
Gene Name
Product ID
S-KO-20013
Gene Alias
Fmnl, Fnrl, Frls, 8030453N10Rik
Background
C57BL/6JCya
Gene Full Name
formin-like 1
Modification
Conventional knockout
NCBI ID
Phenotype
Chromosome
Chr 11 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000042286
NCBI Transcript ID
NM_001077698
Target Region
Exon 4~6
Size of Effective Region
~1.9 kb
Overview of Gene Research
Fmnl1, or Formin-like 1, belongs to the formin protein family responsible for cytoskeletal remodeling. It has been implicated in various cellular processes, such as cell migration, proliferation, and invasion, by interacting with signaling pathways like CXCR2, TGF-β1/SMADs, and Rac1 [1-3]. Fmnl1 is expressed in multiple cell types, including hematopoietic cells, T cells, and cancer cells, and is crucial for normal immune function and has significant implications in cancer progression. Genetic models, including gene-knockout (KO) models, are valuable for studying its functions.
In cancer, FMNL1 depletion in multiple cancer cell lines and in vivo models has shown significant impacts. In clear cell renal cell carcinoma (ccRCC), FMNL1 upregulation promotes metastasis via induction of CXCR2, and high expression is associated with poor prognosis. Knockdown of FMNL1 inhibits cell migration in vitro and tumor metastasis in vivo [1]. In non-small cell lung cancer (NSCLC), reducing FMNL1 expression suppresses cell proliferation, migration, invasion, and bone metastasis by reducing TGF-β1 expression [2]. In leukemia cells, FMNL1 depletion decreases cell proliferation, colony formation, migration, and tumor growth of xenografts, and its silencing increases Rac1 activity [3]. In nasopharyngeal carcinoma, depletion of FMNL1 suppresses invadopodia formation, epithelial-mesenchymal transition (EMT), and invasive/metastatic capacities [4]. In glioblastoma multiforme, FMNL1 downregulation suppresses cell migration and invasion, actin fiber assembly, and is an independent predictor of poor prognosis [5]. In breast adenocarcinoma, suppression of FMNL1 protein expression impairs cell adhesion, migration, and invasion [6].
In conclusion, Fmnl1 plays essential roles in cell migration, proliferation, and invasion, with significant implications in cancer progression. KO and other loss-of-function models have been instrumental in revealing these roles, highlighting Fmnl1 as a potential prognostic factor and therapeutic target in multiple cancer types. Additionally, Fmnl1 is associated with T cell migration in the immune system, indicating its broader importance in biological processes [1-4, 6, 7, 9].
References：
1. Zhang, Mei-Fang, Li, Qiu-Li, Yang, Yu-Feng, Cao, Yun, Zhang, Chris Zhiyi. 2020. FMNL1 Exhibits Pro-Metastatic Activity via CXCR2 in Clear Cell Renal Cell Carcinoma. In Frontiers in oncology, 10, 564614. doi:10.3389/fonc.2020.564614. https://pubmed.ncbi.nlm.nih.gov/33324547/
2. Yang, Xing-Yi, Liao, Jun-Jie, Xue, Wu-Rong. 2019. FMNL1 down-regulation suppresses bone metastasis through reducing TGF-β1 expression in non-small cell lung cancer (NSCLC). In Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 117, 109126. doi:10.1016/j.biopha.2019.109126. https://pubmed.ncbi.nlm.nih.gov/31387165/
3. Favaro, Patricia, Traina, Fabiola, Machado-Neto, João Agostinho, Ridley, Anne J, Saad, Sara Teresinha Olalla. 2013. FMNL1 promotes proliferation and migration of leukemia cells. In Journal of leukocyte biology, 94, 503-12. doi:10.1189/jlb.0113057. https://pubmed.ncbi.nlm.nih.gov/23801653/
4. Chen, Wen-Hui, Cai, Mu-Yan, Zhang, Jia-Xing, Qian, Chao-Nan, Xie, Dan. 2018. FMNL1 mediates nasopharyngeal carcinoma cell aggressiveness by epigenetically upregulating MTA1. In Oncogene, 37, 6243-6258. doi:10.1038/s41388-018-0351-8. https://pubmed.ncbi.nlm.nih.gov/30013189/
5. Higa, Nayuta, Shinsato, Yoshinari, Kamil, Muhammad, Yoshimoto, Koji, Arita, Kazunori. 2019. Formin-like 1 (FMNL1) Is Associated with Glioblastoma Multiforme Mesenchymal Subtype and Independently Predicts Poor Prognosis. In International journal of molecular sciences, 20, . doi:10.3390/ijms20246355. https://pubmed.ncbi.nlm.nih.gov/31861134/
6. Miller, Eric W, Blystone, Scott D. 2019. The carboxy-terminus of the formin FMNL1ɣ bundles actin to potentiate adenocarcinoma migration. In Journal of cellular biochemistry, 120, 14383-14404. doi:10.1002/jcb.28694. https://pubmed.ncbi.nlm.nih.gov/30977161/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
