NKG-hIL6 Mouse
Product Name
NKG-hIL6 Mouse
Product ID
I001176
Strain Name
NOD.Cg-PrkdcscidIl2rgem1Il6em1(hIL6)/Cya
Backgroud
NKG
Status
When using this mouse strain in a publication, please cite “NKG-hIL6 Mouse (Catalog I001176) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Gene Name
IL6 & Il2rg
Gene Alias
CDF, HGF, HSF, BSF2, IL-6, BSF-2, IFNB2, IFN-beta-2, gc, p64, [g]c, CD132, gamma(c)
NCBI ID
Chromosome
Chr 7 (Human), Chr X (Mouse)
Datasheet
Strain Description
NKG mice are a type of severe immunodeficient mouse developed by Cyagen by deleting the Il2rg gene from the NOD-Scid strain. This strain lacks mature T, B, and NK cells, exhibits reduced complement activity, and weak macrophage phagocytosis of human cells. As a result, NKG mice can efficiently engraft human hematopoietic stem cells (HSC), peripheral blood mononuclear cells (PBMC), patient-derived xenografts (PDX), or adult stem cells and tissues.
In immunology research, direct studies on mice may not fully represent the human immune system due to physiological and immune system differences. However, by transplanting human peripheral blood mononuclear cells (PBMCs) or hematopoietic stem cells (HSCs) into immunodeficient mice, we can partially or completely replace the mouse immune system with a human counterpart. This approach enables in vivo simulation of human immune system function, providing an effective model for studying human immunity. In practical human-mouse xenotransplantation, using standard immunodeficient mice for transplantation may lead to differences in transplant efficiency due to the absence of specific human growth factors and supportive stromal cells within the mouse. Genetically modifying immunodeficient mice through gene editing techniques is a common strategy to enhance xenotransplantation efficiency.
Interleukin-6 (IL-6) is a cytokine that plays a crucial role in inflammation and B cell maturation. It is primarily produced and secreted into the bloodstream at acute and chronic inflammatory sites. IL-6 induces transcriptional inflammatory responses through its receptor, IL6Rα. Research indicates that immunodeficient mice carrying the human IL6 gene effectively enhance the differentiation of human monocytes and macrophages during HSC reconstruction [1]. NKG-hIL6 mice were constructed by introducing the human IL6 gene into the genome of NKG mice. Compared to NKG mice, NKG-hIL6 mice can efficiently and rapidly rebuild human CD45+ leukocytes during HSC reconstruction. These mice are valuable for developing tumor immunotherapies and drug evaluations.
Reference
Hanazawa A, Ito R, Katano I, Kawai K, Goto M, Suemizu H, Kawakami Y, Ito M, Takahashi T. Generation of Human Immunosuppressive Myeloid Cell Populations in Human Interleukin-6 Transgenic NOG Mice. Front Immunol. 2018 Feb 2;9:152.
Strain Strategy
The sequences from ATG start codon to TAG stop codon of the endogenous mouse Il6 gene (NKG background) will be replaced with the sequences from ATG start codon to TAG stop codon of the human IL6 gene.
Figure 1. Diagram of the gene editing strategy for the generation of NKG-hIL6 mice.
Application Area
Construction of an immune system humanized mouse model;
Research on the human immune system and hematopoietic systems;
Human-derived cell line xenograft (CDX) and patient-derived xenograft (PDX).
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