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Citations
IMMUNITY 47:107 (2017) IF=22.845
Sftpc-iCre Mice
Catalog Number：C001359
Genetic Background： C57BL/6J
Reproduction：Heterozygote x WT
Strain Description
The Sftpc gene encodes pulmonary surfactant protein C (SPC), an extremely hydrophobic surfactant protein that is essential for lung function and postnatal homeostasis in vivo. The Sftpc gene encodes a 197-amino-acid surfactant protein precursor that is subsequently cleaved to form mature surfactant protein C (SP-C). SP-C is incorporated into lamellipodia in type II alveolar cells along with specific phospholipids and proteins. It is released through regulated exocytosis at the alveolar air-liquid interface and plays a key role in regulating lung mechanics.
When Sftpc-iCre mice are crossed with mice containing loxP sites, the offspring can generate sequence recombination between loxP sites mediated by Cre recombinase in type II alveolar cells. Both homozygous and heterozygous Sftpc-iCre mice are viable and fertile.
The “IRES-iCre” cassette was inserted into the 3’UTR (exon 6).
a. Method
The Sftpc-iCre mice were bred with Rosa26-LSL-tdTomato mice to generate double heterozygous offspring. Cre-mediated recombination will result in the expression of tdTomato protein in the Cre-positive cells of the offspring. The lung tissue was collected from the offspring mice and frozen sections and immunohistochemistry were used to observe the expression of tdTomato protein to determine the activity of Cre recombinase.
b. Genotype
Cre+: Sftpc-iCre[KI/+]; Rosa26-LSL-tdTomato[CKI/+]
Cre-: Rosa26-LSL-tdTomato[CKI/+]
| Fluorescent signal | DAPI | tdTomato |
|---|---|---|
| Exposure time | 2ms | 50ms |
c. Result
1. Occurrence of fluorescence in the lungs
Figure 1. Occurrence of fluorescence in the lungs. The results showed that strong tdTomato orange fluorescence signals were detected in the corresponding cells in the lungs.
Notes：
(1) The fluorescence was observed using the orange light segment as mentioned above.
dTomato peak: excitation 554, emission 581.
Orange light wavelength range: 590-610nm. Red light wavelength range: 625-740nm.
2. Immunohistochemistry of the lung tissues
Figure 2. Immunohistochemistry of the lung tissues. The results showed that the corresponding cells in the lungs displayed significant tdTomato signals.
In summary, this model shows significant fluorescence recombination in type II alveolar cells.
The Cre recombinase gene is located on chromosome 14 in Sftpc-iCre mice. Please avoid using mice that have been targeted on the same chromosome for breeding.
