This strain is a Mecp2 gene knockout (KO) mouse model of Rett syndrome generated by disrupting the Mecp2 gene (the murine ortholog of the human MECP2 gene) on the X chromosome using genome-editing techniques. Studies have demonstrated that Mecp2 KO mice exhibit phenotypes closely resembling those of human RTT, including shortened lifespan, motor impairments, abnormal breathing, stereotypic behaviors, anxiety, social interaction deficits, and cognitive impairments [4]. These features make the Mecp2 KO mouse a valuable tool for studying the pathogenesis of RTT, drug screening, and the development of gene therapies in preclinical research. Preliminary validation data indicate that this strain exhibits abnormalities in body weight and survival rates, along with progressive RTT phenotypes such as gait defects, hindlimb clasping, tremors, motor dysfunction, and respiratory abnormalities.