hIL1RAP Mouse
Product Name
hIL1RAP Mouse
Product ID
C001631
Strain Name
C57BL/6NCya-Il1rapem1(hIL1RAP)/Cya
Backgroud
C57BL/6NCya
Status
When using this mouse strain in a publication, please cite “hIL1RAP Mouse (Catalog C001631) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Gene Name
IL1RAP
Gene Alias
IL1R3, C3orf13, IL-1RAcP
NCBI ID
Chromosome
Chr 3 (Human)
MGI ID
Datasheet
Strain Description
The interleukin-1 receptor accessory protein, encoded by IL1RAP, serves as a critical co-receptor for multiple cytokine systems, notably the interleukin-1 (IL-1) family. This broadly expressed protein is essential for the formation of functional receptor complexes for IL-1, IL-33, and IL-36, thereby mediating downstream signaling pathways that drive innate immunity and inflammatory responses [1-5]. Acting as a co-receptor, IL1RAP interacts with ligand-binding receptors such as IL1R1, IL33R (IL1RL1/ST2), and IL36R, initiating MyD88-dependent signal transduction that culminates in the expression of pro-inflammatory genes and cytokines [1-5]. IL1RAP expression is observed across a range of cell types, including immune, endothelial, epithelial, and neuronal cells, as well as various tumor lineages. Perturbations in IL1RAP expression or genetic variants have been implicated in a spectrum of pathologies, encompassing inflammatory diseases such as asthma, autoimmune disorders, neurodegenerative conditions such as Alzheimer's disease, and malignancies including acute myeloid leukemia, Ewing sarcoma, pancreatic cancer, and gastric adenocarcinoma [2-4]. Consequently, IL1RAP represents a target of intense investigation for therapeutic interventions in cancer and inflammatory diseases [4-5].
hIL1RAP mice are humanized models generated using gene editing technology. They are constructed by integrating the protein-coding sequence (CDS) encoding the extracellular domain of human IL1RAP protein and the intracellular domain of mouse IL1RAP protein, along with the 3'UTR of the mouse Il1rap gene, into a specific site within the mouse Il1rap gene. This process retains the endogenous gene sequence encoding the mouse signal peptide. Homozygous hIL1RAP mice are viable and fertile. This model can be used for studying the pathological mechanisms and therapeutic approaches of cancer and inflammatory diseases, and for the development of IL1RAP-targeted drugs.
Reference
Frenay J, Bellaye PS, Oudot A, Helbling A, Petitot C, Ferrand C, Collin B, Dias AMM. IL-1RAP, a Key Therapeutic Target in Cancer. Int J Mol Sci. 2022 Nov 29;23(23):14918.
Wu M, Zheng X, Huang J, Hu X. Association of IL33, IL1RL1, IL1RAP Polymorphisms and Asthma in Chinese Han Children. Front Cell Dev Biol. 2021 Dec 15;9:759542.
Zettergren A, Höglund K, Kern S, Thorvaldsson V, Johan Skoog M, Hansson O, Andreasen N, Bogdanovic N, Blennow K, Skoog I, Zetterberg H. Association of IL1RAP-related genetic variation with cerebrospinal fluid concentration of Alzheimer-associated tau protein. Sci Rep. 2019 Feb 21;9(1):2460.
Zarezadeh Mehrabadi A, Shahba F, Khorramdelazad H, Aghamohammadi N, Karimi M, Bagherzadeh K, Khoshmirsafa M, Massoumi R, Falak R. Interleukin-1 receptor accessory protein (IL-1RAP): A magic bullet candidate for immunotherapy of human malignancies. Crit Rev Oncol Hematol. 2024 Jan;193:104200.
Zarezadeh Mehrabadi A, Aghamohamadi N, Khoshmirsafa M, Aghamajidi A, Pilehforoshha M, Massoumi R, Falak R. The roles of interleukin-1 receptor accessory protein in certain inflammatory conditions. Immunology. 2022 May;166(1):38-46.
Strain Strategy
The partial coding sequence of exon 2 to partial intron 2 sequence of the mouse Il1rap gene was replaced with an "Extracellular domain of Human IL1RAP CDS-Mouse Il1rap CDS-3'UTR of Mouse Il1rap-WPRE-BGH pA" cassette. The murine signal peptide of the mouse IL1RAP protein was preserved.
Figure 1. Gene editing strategy of hIL1RAP mice.
Application Area
IL1RAP-targeted drug screening, development, and evaluation;
Research on the pathological mechanisms and therapeutic approaches of cancer and inflammatory diseases.
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