huTP53 Mouse
Product Name
huTP53 Mouse
Product ID
C001640
Strain Name
C57BL/6JCya-Trp53tm3(hTP53)/Cya
Backgroud
C57BL/6JCya
Status
When using this mouse strain in a publication, please cite “huTP53 Mouse (Catalog C001640) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Gene Name
TP53
Gene Alias
P53, BCC7, LFS1, BMFS5, TRP53
NCBI ID
Chromosome
Chr 17 (Human)
MGI ID
Datasheet
Strain Description
The TRP53 (TP53 in humans) gene is a critical tumor suppressor gene that encodes the p53 protein, often called the "guardian of the genome." This protein is expressed at low levels in normal cells but becomes highly activated in response to various cellular stresses like DNA damage, hypoxia, and oncogene activation [1]. The p53 protein is a transcription factor that regulates a vast network of target genes, directing the cell's response to stress by inducing cell cycle arrest, DNA repair, apoptosis (programmed cell death), or senescence (permanent growth arrest). Its activity is crucial for maintaining genomic stability and preventing malignant transformation [2]. Mutations in TP53 are the most frequent genetic alterations in human cancers, found in over 50% of all malignancies. These mutations not only cause a loss of the protein's tumor-suppressing function but can also confer new, "gain-of-function" oncogenic properties that promote tumor growth, invasion, and metastasis. While most commonly associated with a wide range of cancers, including hereditary cancers like Li-Fraumeni syndrome, abnormal p53 activity has also been linked to non-neoplastic conditions such as certain developmental and neurodegenerative disorders, like Alzheimer's and Huntington's disease, and bone marrow failure syndrome. The p53 protein is expressed ubiquitously across most cellular tissues due to its fundamental role in cellular homeostasis [3].
huTP53 mouse is a humanized model constructed via gene editing technology, in which the region from ATG start codon to downstream of 3'UTR of mouse Trp53 is replaced with the region from ATG start codon to downstream of 3'UTR of human TP53. Initial research has shown that breeding the homozygous line results in a very small number of pups. While heterozygous females can breed normally, their maternal instincts are poor, with instances of cannibalism and occasional deaths of pups due to a failure to thrive. huTP53 mouse can be used for mechanistic research on a wide range of cancers, including hereditary cancers like Li-Fraumeni syndrome and preclinical studies of TP53-targeted drugs.
Reference
Tornesello ML. TP53 mutations in cancer: Molecular features and therapeutic opportunities (Review). Int J Mol Med. 2025 Jan;55(1):7.
Sammons MA, Nguyen TT, McDade SS, Fischer M. Tumor suppressor p53: from engaging DNA to target gene regulation. Nucleic Acids Res. 2020 Sep 18;48(16):8848-8869.
Liu Y, Su Z, Tavana O, Gu W. Understanding the complexity of p53 in a new era of tumor suppression. Cancer Cell. 2024 Jun 10;42(6):946-967.
Strain Strategy
The region from the ATG start codon to downstream of the 3'UTR of mouse Trp53 was replaced with the region from the ATG start codon to downstream of the 3'UTR of human TP53.
Figure 1. Gene editing strategy of huTP53 mice.
Application Area
Screening, development, and preclinical evaluation of TP53-targeted drugs;
Mechanistic research on a wide range of cancers, including hereditary cancers like Li-Fraumeni syndrome, and evaluation of therapeutic drugs.
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