The Rho KO mouse is a gene knockout model created using gene-editing techniques to knock out the coding sequence of the Rho gene (the homolog of the human RHO gene) in mice. The validation data shows that this model exhibits significant thinning of the outer nuclear layer (ONL) of the retina and abnormalities in electroretinography (ERG), effectively mimicking human disease phenotypes. Therefore, it can be used to study the pathogenic mechanisms of retinitis pigmentosa and congenital stationary night blindness, as well as for the research and development of related therapeutic interventions.