B6-hIL2RA Mouse
Product Name
B6-hIL2RA Mouse
Product ID
C001713
Strain Name
C57BL/6NCya-Il2ratm1(hIL2RA)/Cya
Backgroud
C57BL/6NCya
Status
When using this mouse strain in a publication, please cite “B6-hIL2RA Mouse (Catalog C001713) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Gene Name
IL2RA
Gene Alias
p55, CD25, IL2R, IMD41, TCGFR, IDDM10
NCBI ID
Chromosome
Chr 10 (Human)
MGI ID
Datasheet
Strain Description
The interleukin-2 receptor alpha subunit, encoded by the IL2RA gene and also known as CD25, is a critical determinant of IL-2 signaling, a pathway fundamental to T cell biology. While CD25 alone exhibits low affinity for IL-2, its assembly with the IL-2 receptor beta and gamma chains forms the high-affinity receptor complex essential for robust cellular responses to this pleiotropic cytokine [1]. Expressed prominently on activated T lymphocytes, including effector and regulatory T cells, CD25 is pivotal for diverse processes such as T cell proliferation, differentiation, and the maintenance of immune tolerance, largely mediated through its indispensable role in regulatory T cell development and function [2]. Consequently, perturbations in IL2RA expression or genetic variants within the locus are strongly associated with susceptibility to a range of severe autoimmune disorders, including multiple sclerosis, type 1 diabetes, and rheumatoid arthritis, highlighting its central involvement in immune homeostasis breakdown [3]. Furthermore, aberrant CD25 expression has been observed in certain malignancies, suggesting roles beyond adaptive immunity [4]. The demonstrable impact of IL2RA on immune regulation and disease pathogenesis underscores its significance as a key molecule in immunology and a compelling target for therapeutic intervention.
The B6-hIL2RA mouse is a humanized model constructed by replacing the sequence of the mouse Il2ra endogenous extracellular domain in situ with the corresponding extracellular domain from the human IL2RA. The murine signal peptide and transmembrane-cytoplasmic region were preserved. The B6-hIL2RA mice can be used for the study of the pathogenesis of autoimmune diseases such as multiple sclerosis, type 1 diabetes, and rheumatoid arthritis, and certain malignancies, as well as for IL2RA-targeted drug development.
Reference
Ross SH, Cantrell DA. Signaling and Function of Interleukin-2 in T Lymphocytes. Annu Rev Immunol. 2018 Apr 26;36:411-433.
Rangani F, Rakhshi N, Kadkhoda Mezerji Z, Alikhah A, Dehghanzad R, Abbasi B, Ahmadi A, Nikravesh A, Pahlevan Kakhki M. Association of IL2RA and multiple sclerosis risk: A case control, systematic review, and meta-analysis study. J Neurol Sci. 2025 May 15;472:123461.
Nguyen CH, Schlerka A, Grandits AM, Koller E, van der Kouwe E, Vassiliou GS, Staber PB, Heller G, Wieser R. IL2RA Promotes Aggressiveness and Stem Cell-Related Properties of Acute Myeloid Leukemia. Cancer Res. 2020 Oct 15;80(20):4527-4539.
Fan L, Wang X, Chang Q, Wang Y, Yang W, Liu L. IL2RA is a prognostic indicator and correlated with immune characteristics of pancreatic ductal adenocarcinoma. Medicine (Baltimore). 2022 Oct 21;101(42):e30966.
Strain Strategy
Figure 1. Gene editing strategy of B6-hIL2RA Mice. The mouse Il2ra endogenous extracellular domain was replaced with the human IL2RA extracellular domain. The murine signal peptide and transmembrane-cytoplasmic region were preserved.
Application Area
IL2RA-targeted drug screening, development, and evaluation;
Research on the pathological mechanisms and therapeutic approaches of autoimmune diseases such as multiple sclerosis, type 1 diabetes, and rheumatoid arthritis.
Tumor immunology and other anti-tumor research.
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