B6-hIL2 Mouse
Product Name
B6-hIL2 Mouse
Product ID
C001804
Strain Name
C57BL/6NCya-Il2tm1(hIL2)/Cya
Backgroud
C57BL/6NCya
Status
When using this mouse strain in a publication, please cite “B6-hIL2 Mouse (Catalog C001804) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Gene Name
IL2
Gene Alias
IL-2, TCGF, lymphokine
NCBI ID
Chromosome
Chr 4 (Human)
MGI ID
Datasheet
Strain Description
The IL2 gene encodes Interleukin-2, a crucial cytokine primarily secreted by activated CD4+ and CD8+ T lymphocytes, and to a lesser extent, by natural killer (NK) cells. This protein, also known as T-cell growth factor, plays a pivotal role in regulating immune responses by promoting the growth, proliferation, and differentiation of various "disease-fighting blood cells", including T cells, B cells, NK cells, monocytes, macrophages, and oligodendrocytes [1]. IL2 expression is tightly regulated; it is upregulated upon T-cell activation through T-cell receptor signaling and can be inhibited by factors like TOB and TGF-beta [2]. The encoded IL-2 protein mediates its effects by binding to the IL-2 receptor (IL-2R), a complex found on lymphocytes, which then activates downstream signaling pathways such as JAK-STAT, PI3K/Akt/mTOR, and MAPK/ERK, influencing T-cell survival, differentiation, and the maintenance of immune tolerance by supporting regulatory T cells (Tregs) while inhibiting pro-inflammatory Th17 cell differentiation [3]. Deregulation of IL2 function, whether due to deficiency or excess, is implicated in various autoimmune diseases, including systemic lupus erythematosus (SLE), rheumatoid arthritis (RA), systemic sclerosis, Sjögren's syndrome, and multiple sclerosis, as well as certain cancers like metastatic melanoma, renal cell carcinoma, and non-Hodgkin lymphoma [4].
The B6-hIL2 mouse is a humanized model, constructed by replacing the coding sequences of the endogenous mouse Il2 gene with the coding sequences of the human IL2 gene. B6-hIL2 mice can be used for research into the pathogenesis of autoimmune disorders like systemic lupus erythematosus (SLE), rheumatoid arthritis (RA), systemic sclerosis, Sjögren's syndrome, multiple sclerosis, and type 1 diabetes, as well as for the screening, development, and safety evaluation of IL2-targeted drugs.
Reference
Liao W, Lin JX, Leonard WJ. Interleukin-2 at the crossroads of effector responses, tolerance, and immunotherapy. Immunity. 2013 Jan 24;38(1):13-25.
Das L, Levine AD. TGF-beta inhibits IL-2 production and promotes cell cycle arrest in TCR-activated effector/memory T cells in the presence of sustained TCR signal transduction. J Immunol. 2008 Feb 1;180(3):1490-8.
Ross SH, Cantrell DA. Signaling and Function of Interleukin-2 in T Lymphocytes. Annu Rev Immunol. 2018 Apr 26;36:411-433.
Rafaqat S. Role of IL-2/IL-2 receptor in pathogenesis of autoimmune disorders: Genetic and therapeutic aspects. World J Med Genet. 2023 Jul 20;11(3):28-38.
Strain Strategy
The sequences from the ATG start codon to the TAA stop codon of the endogenous mouse Il2 gene were replaced with the sequences from the ATG start codon to the TGA stop codon of the human IL2 gene.
Figure 1. Gene editing strategy of B6-hIL2 mice.
Application Area
IL2-targeted drug screening, development, and evaluation;
Research on the pathological mechanisms and therapeutic approaches of certain cancers;
Research on the pathological mechanisms and therapeutic approaches of autoimmune disorders, such as systemic lupus erythematosus (SLE), rheumatoid arthritis (RA), systemic sclerosis, Sjögren's syndrome, multiple sclerosis, and type 1 diabetes.
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