B6-hMRGPRX2 Mouse
Product Name
B6-hMRGPRX2 Mouse
Product ID
C001846
Strain Name
C57BL/6NCya-Mrgprx2tm1(hMRGPRX2)/Cya
Backgroud
C57BL/6NCya
Status
When using this mouse strain in a publication, please cite “B6-hMRGPRX2 Mouse (Catalog C001846) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Gene Name
MRGPRX2
Gene Alias
MGRG3, MRGX2
NCBI ID
Chromosome
Chr 11 (Human)
MGI ID
Datasheet
Strain Description
The MRGPRX2 gene, a member of the G protein-coupled receptor (GPCR) family, encodes a unique receptor protein with a pivotal role in immunological and neurological signaling [1]. Primarily expressed on mast cells and sensory neurons, the MRGPRX2 protein functions as a non-canonical receptor that circumvents traditional IgE-mediated pathways. Its ligand repertoire is remarkably broad, encompassing a diverse array of stimuli, including endogenous neuropeptides (e.g., substance P) and exogenous cationic drugs (e.g., fluoroquinolones, neuromuscular blocking agents) [2]. Ligand binding to MRGPRX2 initiates mast cell degranulation, a process that releases potent pro-inflammatory mediators such as histamine and tryptase. This activation mechanism is a central driver of pseudo-allergic reactions, a class of drug hypersensitivities that mimic true allergic responses without involving IgE [3]. Emerging evidence further implicates MRGPRX2 in the pathophysiology of various inflammatory and pruritic diseases, including chronic spontaneous urticaria and atopic dermatitis, underscoring its therapeutic potential as a target for modulating mast cell activity [4].
The B6-hMRGPRX2 mouse is a humanized model, constructed by replacing the sequences from exon 1 to partial intron 1 of mouse Mrgprx2 with the Kozak-human MRGPRX2 CDS-3'UTR of mouse Mrgprx2-WPRE-BGH pA cassette. B6-hMRGPRX2 mice can be used for research into the pathogenesis of various inflammatory and pruritic diseases, including chronic spontaneous urticaria and atopic dermatitis. They are also useful for the development of MRGPRX2-targeted drugs.
Reference
Guo Y, Ollé L, Proaño-Pérez E, Aparicio C, Guerrero M, Muñoz-Cano R, Martín M. MRGPRX2 signaling involves the Lysyl-tRNA synthetase and MITF pathway. Front Immunol. 2023 May 10;14:1154108.
Subramanian H, Gupta K, Ali H. Roles of Mas-related G protein-coupled receptor X2 on mast cell-mediated host defense, pseudoallergic drug reactions, and chronic inflammatory diseases. J Allergy Clin Immunol. 2016 Sep;138(3):700-710.
Kumar M, Duraisamy K, Chow BK. Unlocking the Non-IgE-Mediated Pseudo-Allergic Reaction Puzzle with Mas-Related G-Protein Coupled Receptor Member X2 (MRGPRX2). Cells. 2021 Apr 27;10(5):1033.
Ogasawara H, Noguchi M. Therapeutic Potential of MRGPRX2 Inhibitors on Mast Cells. Cells. 2021 Oct 27;10(11):2906.
Strain Strategy
The coding sequences of exon 1 plus partial intron 1 of mouse Mrgprx2 were replaced with the Kozak-human MRGPRX2 CDS-3'UTR of mouse Mrgprx2-WPRE-BGH pA cassette.
Figure 1. Gene editing strategy of B6-hMRGPRX2 mice.
Application Area
MRGPRX2-targeted drug screening, development, and evaluation;
Research on the pathological mechanisms and therapeutic approaches of various inflammatory and pruritic diseases, including chronic spontaneous urticaria and atopic dermatitis.
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