B6-huAPOE4 Mouse
Product Name
B6-huAPOE4 Mouse
Product ID
C001867
Strain Name
C57BL/6JCya-Apoetm6(hAPOEε4)/Cya
Backgroud
C57BL/6JCya
Status
When using this mouse strain in a publication, please cite “B6-huAPOE4 Mouse (Catalog C001867) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Gene Name
APOE
Gene Alias
AD2, LPG, APO-E, ApoE4, LDLCQ5
NCBI ID
Chromosome
Chr 19 (Human)
MGI ID
Datasheet
Strain Description
Apolipoprotein E (APOE) is a critical apolipoprotein involved in lipid transport mediated by lipoproteins. As a core component of plasma lipoproteins, APOE facilitates the transport of lipids through plasma and interstitial fluid between organs, and it plays a pivotal role in the generation, conversion, and clearance of lipoproteins. In humans, the APOE gene has three isoforms (E2, E3, E4) associated with atherosclerosis and Alzheimer’s disease (AD), with the E4 allele present in approximately 14% of the population [1]. The ApoE4 isoform is a major genetic risk factor for late-onset Alzheimer’s disease (AD), exacerbating neurodegeneration. ApoE4-associated damage to vascular systems in the brain could have a key role in AD pathogenesis [2]. Beyond AD, APOE4 is linked to cardiovascular diseases due to its influence on lipid homeostasis [3].
B6-huAPOE4 mice are humanized models constructed through gene editing technology. Exons 2-4 plus partial flanking sequences of the mouse Apoe gene were replaced in situ with the Mutant human APOE gene sequence, including exons 2, 3, and 4 and some downstream sequence of 3’UTR. The p.C130R (TGC to CGC) was introduced into the mutant human APOE gene. This model can be used for research on the pathogenic mechanisms and treatment methods of cardiovascular diseases such as diet-induced hypercholesterolemia, atherosclerosis, and lipid metabolism. It can also be used to study the role of human APOE gene polymorphisms in Alzheimer's disease.
Reference
Heffernan AL, Chidgey C, Peng P, Masters CL, Roberts BR. The Neurobiology and Age-Related Prevalence of the ε4 Allele of Apolipoprotein E in Alzheimer's Disease Cohorts. J Mol Neurosci. 2016 Nov;60(3):316-324.
Liu CC, Liu CC, Kanekiyo T, Xu H, Bu G. Apolipoprotein E and Alzheimer disease: risk, mechanisms and therapy. Nat Rev Neurol. 2013 Feb;9(2):106-18. doi: 10.1038/nrneurol.2012.263. Epub 2013 Jan 8. Erratum in: Nat Rev Neurol. 2013.
Mahley RW. Apolipoprotein E: from cardiovascular disease to neurodegenerative disorders. J Mol Med (Berl). 2016 Jul;94(7):739-46.
Strain Strategy
Exons 2-4 plus partial flanking sequences were replaced with the Mutant human APOE gene sequence, including exons 2, 3, and 4 and some downstream sequence of 3’UTR. The p.C130R (TGC to CGC) was introduced into the mutant human APOE gene.
Figure 1. Gene editing strategy of B6-huAPOE4 mice.
Application Area
The screening, development, and preclinical evaluation of APOE4-targeted drugs;
Research on the pathogenic mechanisms and treatment methods of cardiovascular diseases, such as diet-induced hypercholesterolemia, atherosclerosis, and lipid metabolism;
Study the role of human APOE gene polymorphisms in Alzheimer’s disease (AD).
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