huCCL1(BALB/c) Mouse
Product Name
huCCL1(BALB/c) Mouse
Product ID
C001989
Strain Name
BALB/cAnCya-Ccl1tm1(hCCL1)/Cya
Backgroud
BALB/cAnCya
Status
When using this mouse strain in a publication, please cite “huCCL1(BALB/c) Mouse (Catalog C001989) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Gene Name
CCL1
Gene Alias
P500, SISe, TCA3, I-309, SCYA1
NCBI ID
Chromosome
Chr 17 (Human)
MGI ID
Datasheet
Strain Description
C-C motif chemokine ligand 1 (CCL1), also known as I-309, is a small chemokine mainly secreted by activated T cells, monocytes, and endothelial cells. It belongs to the CC chemokine family and plays a key regulatory role in Th2-type immune responses and the recruitment of regulatory T cells (Tregs) [1]. CCL1 binds to its specific receptor CCR8 (C-C chemokine receptor type 8) to mediate the directed migration of Th2 cells and Tregs to inflammatory sites and the tumor microenvironment, promoting the formation of an immunosuppressive tumor microenvironment (TME). It is also involved in the immune regulation of allergic inflammatory responses, autoimmune diseases, and graft-versus-host disease (GvHD) [2-3]. The CCL1 gene is located on human chromosome 17 (17q12) and is closely associated with immune evasion in various solid tumors, Th2-type allergic diseases, and the development of inflammatory bowel disease (IBD). Targeting the CCL1-CCR8 signaling axis has become an important strategy for drug development in tumor immunotherapy, allergic diseases, and autoimmune disorders [4-5].
The huCCL1(BALB/c) mouse is a humanized model constructed through gene editing technology. The sequences from the start codon to the stop codon of the endogenous mouse Ccl1 gene were replaced with the sequences from the start codon to the stop codon of the human CCL1 gene. This model is suitable for evaluating the efficacy and safety of CCL1-targeted antibodies and CAR-T/CAR-NK cell therapies, as well as for preclinical research in tumor immunity, Th2/Treg immune regulation, allergic disorders, and autoimmune diseases.
Reference
Miller MD, Krangel MS. The human cytokine I-309 is a monocyte chemoattractant. Proc Natl Acad Sci USA, 1992; 89(7): 2950–2954.
Tiffany HL, Lautens LL, Gao JL, et al. Identification of CCR8: a human monocyte and thymus receptor for the CC chemokine I-309. J Exp Med, 1997; 186(1): 165–170.
Zingoni A, Soto H, Hedrick JA, et al. The chemokine receptor CCR8 is preferentially expressed in Th2 but not Th1 cells. J Immunol, 1998; 161(2): 547–551.
Haque NS, Fallon JT, Pan JJ, et al. Chemokine receptor-8 (CCR8) mediates human vascular smooth muscle cell chemotaxis and metalloproteinase-2 secretion. Blood, 2004; 103(4): 1296–1304.
Eruslanov EB, Bhojnagarwala PS, Quatromoni JG, et al. Tumor-associated neutrophils stimulate T cell responses in early-stage human lung cancer. J Clin Invest, 2014; 124(12): 5466–5480.
Strain Strategy
The sequences from the start codon to the stop codon of the endogenous mouse Ccl1 gene were replaced with the sequences from the start codon to the stop codon of the human CCL1 gene.
Figure 1. Gene editing strategy of huCCL1(BALB/c) mice.
Application Area
Screening, development, and preclinical evaluation of CCL1-targeted therapeutics;
Immune and inflammatory research;
Tumor immunotherapy and microenvironment research;
Autoimmune disease research;
Chemokine signaling pathway research;
Evaluation of antibody therapeutics and small-molecule inhibitors.
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