The SD-Rosa-hAGT rat is a humanized model created by integrating the human AGT gene into the ROSA26 safe harbor locus in rats. The humanized region includes AGT gene introns, exons, 5’ UTR, and 3’ UTR, making it suitable for most drug screening studies targeting AGT. This model can be used for investigating the pathogenic mechanisms and drug development related to primary hypertension, pre-eclampsia, renal tubular dysplasia, non-familial structural atrial fibrillation, and inflammatory bowel disease.