SD-huNPR1 Rat
Product Name
SD-huNPR1 Rat
Product ID
CR010
Strain Name
SD-Npr1em1(hNPR1)/Cya
Backgroud
Sprague-Dawley
Status
When using this mouse strain in a publication, please cite “SD-huNPR1 Rat (Catalog CR010) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Gene Name
NPR1
Gene Alias
ANPa, NPRA, ANPRA, GUC2A, GUCY2A
NCBI ID
Chromosome
Chr 1 (Human)
MGI ID
Datasheet
Strain Description
The human NPR1 gene, also known as natriuretic peptide receptor 1 or guanylyl cyclase-A (GC-A), encodes for a transmembrane receptor called natriuretic peptide receptor-A (NPRA). This protein acts as a cell-surface receptor for atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP), two key hormones that regulate cardiovascular homeostasis [1]. Upon binding, NPRA catalyzes the conversion of GTP to the second messenger cyclic GMP (cGMP), leading to vasodilation, natriuresis (increased sodium excretion), diuresis (increased urine production), and protection against cardiac remodeling [1-2]. The NPR1 gene is expressed in various cellular tissues, including the heart, kidneys, vascular smooth muscle, and immune cells. Genetic variations and altered expression of NPR1 are associated with several cardiovascular diseases, including essential hypertension, left ventricular hypertrophy, congestive heart failure, and coronary artery disease [3]. Alterations in NPRA expression have also been linked to certain cancers, such as gastric cancer, and metabolic disorders [4].
SD-huNPR1 rats are a humanized model generated using gene editing technology, in which the rat Npr1 endogenous extracellular domain is replaced with the human NPR1 extracellular domain. SD-huNPR1 rats can be used for studying the pathological mechanisms and therapeutic approaches for several cardiovascular diseases, including essential hypertension, left ventricular hypertrophy, congestive heart failure, and coronary artery disease, as well as specific cancers. They are also useful for the development of NPR1-targeted drugs.
Reference
Misono KS, Philo JS, Arakawa T, Ogata CM, Qiu Y, Ogawa H, Young HS. Structure, signaling mechanism and regulation of the natriuretic peptide receptor guanylate cyclase. FEBS J. 2011 Jun;278(11):1818-29.
Hubers SA, Schirger JA, Sangaralingham SJ, Chen Y, Burnett JC Jr, Hodge D, Chen HH. B-type natriuretic peptide and cardiac remodelling after myocardial infarction: a randomised trial. Heart. 2021 Mar;107(5):396-402.
Vandenwijngaert S, Ledsky CD, Lahrouchi N, Khan MAF, Wunderer F, Ames L, Honda T, Diener JL, Bezzina CR, Buys ES, Bloch DB, Newton-Cheh C; CHARGE+ Exome Chip Blood Pressure Consortium, the CHD Exome+ Consortium, the Exome BP Consortium, the GoT2D Consortium, the T2D-GENES Consortium and the UK Biobank CardioMetabolic Consortium BP Working Group. Blood Pressure-Associated Genetic Variants in the Natriuretic Peptide Receptor 1 Gene Modulate Guanylate Cyclase Activity. Circ Genom Precis Med. 2019 Aug;12(8):e002472.
Cao T, Wang S, Qian L, Wu C, Huang T, Wang Y, Li Q, Wang J, Xia Y, Xu L, Wang L, Huang X. NPRA promotes fatty acid metabolism and proliferation of gastric cancer cells by binding to PPARα. Transl Oncol. 2023 Sep;35:101734.
Strain Strategy
Figure 1. Gene editing strategy for SD-huNPR1 rats. The rat Npr1 endogenous extracellular domain was replaced with the human NPR1 extracellular domain.
Application Area
NPR1-targeted drug screening, development, and evaluation;
Research on the pathological mechanisms and therapeutic approaches of several cardiovascular diseases, including essential hypertension, left ventricular hypertrophy, congestive heart failure, and coronary artery disease;
Research on the pathological mechanisms and therapeutic approaches of specific cancers.
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