C57BL/6JCya-Trmt10aem1flox/Cya
Common Name
Trmt10a-flox
Product ID
S-CKO-00722
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-108943-Trmt10a-B6J-VA
Status
When using this mouse strain in a publication, please cite “Trmt10a-flox Mouse (Catalog S-CKO-00722) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Trmt10a-flox
Strain ID
CKOCMP-108943-Trmt10a-B6J-VA
Gene Name
Product ID
S-CKO-00722
Gene Alias
Rnmtd2, Rg9mtd2, 3110023L08Rik
Background
C57BL/6JCya
Gene Full Name
tRNA methyltransferase 10A
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 3 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000162864
NCBI Transcript ID
NM_001348196.1
Target Region
Exon 3~5
Size of Effective Region
~3.1 Kb
Overview of Gene Research
TRMT10A, short for tRNA methyltransferase 10A, is responsible for N1-methylguanosine modification at position nine of various cytoplasmic tRNAs in higher eukaryotes [1,6]. This modification is crucial for maintaining the normal function of tRNAs, which are essential for protein synthesis. It may also be involved in pathways related to mitochondrial and glucose metabolism [2].
Trmt10a null mice showed a universal decrease in tRNAGln(CUG) and initiator methionine tRNA levels in various tissues, similar to human cell lines lacking TRMT10A. Ribosome profiling of mouse brain revealed that TRMT10A dysfunction causes ribosome slowdown at the Gln(CAG) codon and increased translation of Atf4. In the mutant brain, the translation of a subset of mRNAs, especially those for neuronal structures, is perturbed. Despite no discernable defects in the pancreas, liver, or kidney, Trmt10a null mice had lower body weight, smaller hippocampal postsynaptic densities, defective synaptic plasticity, and memory [1].
In conclusion, TRMT10A plays a vital role in maintaining normal tRNA function and levels, especially for tRNAGln and initiator methionine tRNAs. Through gene-knockout mouse models, it has been revealed that TRMT10A is crucial for normal brain functions, including synaptic plasticity and memory. Dysfunction of TRMT10A is associated with various human diseases such as intellectual disability, microcephaly, diabetes, and short stature [1,2,3,4,5,7].
References：
1. Tresky, Roland, Miyamoto, Yuta, Nagayoshi, Yu, Tomizawa, Kazuhito, Chujo, Takeshi. . TRMT10A dysfunction perturbs codon translation of initiator methionine and glutamine and impairs brain functions in mice. In Nucleic acids research, 52, 9230-9246. doi:10.1093/nar/gkae520. https://pubmed.ncbi.nlm.nih.gov/38950903/
2. Ceraolo, Graziana, Spoto, Giulia, Butera, Ambra, Nicotera, Antonio Gennaro, Di Rosa, Gabriella. 2024. New Insights Into TRMT10A Syndrome: Case Report and Literature Review. In American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics, 198, e33015. doi:10.1002/ajmg.b.33015. https://pubmed.ncbi.nlm.nih.gov/39440920/
3. Samhani, C, Guerci, B, Larose, C. 2024. Discovery of a TRMT10A mutation in a case of atypical diabetes: Case report. In Diabetes & metabolism, 50, 101572. doi:10.1016/j.diabet.2024.101572. https://pubmed.ncbi.nlm.nih.gov/39243962/
4. Stern, Eve, Vivante, Asaf, Barel, Ortal, Levy-Shraga, Yael. 2021. TRMT10A Mutation in a Child with Diabetes, Short Stature, Microcephaly and Hypoplastic Kidneys. In Journal of clinical research in pediatric endocrinology, 14, 227-232. doi:10.4274/jcrpe.galenos.2020.2020.0265. https://pubmed.ncbi.nlm.nih.gov/33448213/
5. Şıklar, Zeynep, Kontbay, Tuğba, Colclough, Kevin, Patel, Kashyap A., Berberoğlu, Merih. 2021. Expanding the Phenotype of TRMT10A Mutations: Case Report and a Review of the Existing Cases. In Journal of clinical research in pediatric endocrinology, 15, 90-96. doi:10.4274/jcrpe.galenos.2021.2021.0110. https://pubmed.ncbi.nlm.nih.gov/34541035/
6. Vilardo, Elisa, Amman, Fabian, Toth, Ursula, Helm, Mark, Rossmanith, Walter. . Functional characterization of the human tRNA methyltransferases TRMT10A and TRMT10B. In Nucleic acids research, 48, 6157-6169. doi:10.1093/nar/gkaa353. https://pubmed.ncbi.nlm.nih.gov/32392304/
7. Lin, Hu, Zhou, Xuelian, Chen, Xuefeng, Polychronakos, Constantin, Dong, Guan-Ping. . tRNA methyltransferase 10 homologue A (TRMT10A) mutation in a Chinese patient with diabetes, insulin resistance, intellectual deficiency and microcephaly. In BMJ open diabetes research & care, 8, . doi:10.1136/bmjdrc-2020-001601. https://pubmed.ncbi.nlm.nih.gov/33067246/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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