C57BL/6JCya-Runx2em1flox/Cya
Common Name:
Runx2-flox
Product ID:
S-CKO-01564
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Runx2-flox
Strain ID
CKOCMP-12393-Runx2-B6J-VA
Gene Name
Product ID
S-CKO-01564
Gene Alias
AML3; CBF-alpha-1; Cbf; Cbfa-1; Cbfa1; LS3; Osf2; PEBP2aA; Pebp2a1; Pebpa2a
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
17
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Runx2em1flox/Cya mice (Catalog S-CKO-01564) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000113571
NCBI RefSeq
NM_001146038
Target Region
Exon 4
Size of Effective Region
~0.7 kb
Detailed Document
Overview of Gene Research
Runx2, also known as Runt-related transcription factor 2, is a crucial transcription factor for skeletal development. It is expressed in multipotent mesenchymal cells, osteoblast-lineage cells, and chondrocytes. Runx2 plays a major role in chondrocyte maturation, osteoblast differentiation, and osteoprogenitor proliferation. It regulates chondrocyte proliferation by directly regulating Ihh expression and is involved in determining the type of cartilage formed. Runx2 also functions in multiple signaling pathways such as hedgehog, Fgf, Wnt, and Pthlh, which are important for the commitment of mesenchymal cells to osteoblast-lineage cells [2,4].
In the context of diseases, SIRT6-transgenic (SIRT6-Tg) mice showed alleviated vascular calcification (VC), while vascular smooth muscle cell-specific (VSMC-specific) SIRT6 knocked-down mice showed severe VC in chronic kidney disease. SIRT6 suppressed the osteogenic transdifferentiation of VSMCs via regulation of Runx2. SIRT6 bound to Runx2, deacetylated it, promoted its nuclear export, and subsequent degradation through the ubiquitin-proteasome system [1]. Also, in the study of cardiac remodeling, Alox5 deficiency significantly ameliorated transverse aortic constriction (TAC)-induced hypertrophy. Alox5 could bind to Runx2, promote its nuclear localization, and contribute to its liquid-liquid phase separation in the nucleus, increasing the transcription of EGFR in cardiomyocytes. Runx2 depletion alleviated hypertrophy in Ang II-pretreated Alox5-overexpressing cardiomyocytes [3].
In conclusion, Runx2 is essential for skeletal development, regulating chondrocyte and osteoblast-lineage cell processes. The gene knockout and conditional knockout mouse models have revealed its role in diseases like vascular calcification and cardiac remodeling. Understanding Runx2 provides insights into the mechanisms of bone-related and certain cardiovascular diseases, which may help in developing new therapeutic strategies [1,3].
References:
1. Li, Wenxin, Feng, Weijing, Su, Xiaoyan, Liu, Baohua, Huang, Hui. . SIRT6 protects vascular smooth muscle cells from osteogenic transdifferentiation via Runx2 in chronic kidney disease. In The Journal of clinical investigation, 132, . doi:10.1172/JCI150051. https://pubmed.ncbi.nlm.nih.gov/34793336/
2. Komori, Toshihisa. . Molecular Mechanism of Runx2-Dependent Bone Development. In Molecules and cells, 43, 168-175. doi:10.14348/molcells.2019.0244. https://pubmed.ncbi.nlm.nih.gov/31896233/
3. Xie, Saiyang, Chen, Mengya, Fang, Wenxi, Deng, Wei, Tang, Qizhu. 2022. Diminished arachidonate 5-lipoxygenase perturbs phase separation and transcriptional response of Runx2 to reverse pathological ventricular remodeling. In EBioMedicine, 86, 104359. doi:10.1016/j.ebiom.2022.104359. https://pubmed.ncbi.nlm.nih.gov/36395739/
4. Komori, Toshihisa. 2024. Regulation of Skeletal Development and Maintenance by Runx2 and Sp7. In International journal of molecular sciences, 25, . doi:10.3390/ijms251810102. https://pubmed.ncbi.nlm.nih.gov/39337587/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen