C57BL/6JCya-Csf1rem1flox/Cya
Common Name:
Csf1r-flox
Product ID:
S-CKO-01902
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Csf1r-flox
Strain ID
CKOCMP-12978-Csf1r-B6J-VA
Gene Name
Product ID
S-CKO-01902
Gene Alias
CD115; CSF-1R; Csfmr; Fim-2; Fim2; Fms; M-CSF-R; M-CSFR
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
18
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Csf1rem1flox/Cya mice (Catalog S-CKO-01902) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000025523
NCBI RefSeq
NM_001037859
Target Region
Exon 3~5
Size of Effective Region
~2.9 kb
Detailed Document
Overview of Gene Research
Csf1r, the Colony-Stimulating Factor-1 Receptor, is a receptor tyrosine kinase. It is crucial for the differentiation and maintenance of most tissue-resident macrophages and bone-resorbing osteoclasts. Activation by cytokine ligation to its ectodomain triggers autophosphorylation of the intracellular tyrosine kinase domain, activating downstream pro-survival kinase cascades like PI3K, ERK1/2, and JNK [1].
In mice, deletion of the fms-intronic regulatory element (FIRE), a super-enhancer in the Csf1r locus, selectively impacts Csf1r expression and tissue macrophage development. Csf1rΔFIRE/ΔFIRE mice lack macrophages in the embryo, brain microglia, and resident macrophages in several tissues, while other macrophage populations and monocytes' homeostasis remain unaffected, except monocytes and their progenitors in bone marrow lacking surface Csf1r [3]. Also, in a mouse model of graft-versus-host disease, CSF1R inhibition led to exacerbated neuroinflammation and behavioral deficits [4].
In conclusion, Csf1r is essential for the development and function of specific macrophage populations. Mouse models with targeted Csf1r modifications, such as the Csf1rΔFIRE/ΔFIRE mice, have revealed its tissue-specific roles. These models also show that Csf1r has implications in diseases like leukoencephalopathy, cancer, and graft-versus-host disease, highlighting its potential as a therapeutic target in these areas [2,1,4].
References:
1. Wen, Jiachen, Wang, Siyuan, Guo, Rongxian, Liu, Dan. 2022. CSF1R inhibitors are emerging immunotherapeutic drugs for cancer treatment. In European journal of medicinal chemistry, 245, 114884. doi:10.1016/j.ejmech.2022.114884. https://pubmed.ncbi.nlm.nih.gov/36335744/
2. Konno, Takuya, Kasanuki, Koji, Ikeuchi, Takeshi, Dickson, Dennis W, Wszolek, Zbigniew K. 2018. CSF1R-related leukoencephalopathy: A major player in primary microgliopathies. In Neurology, 91, 1092-1104. doi:10.1212/WNL.0000000000006642. https://pubmed.ncbi.nlm.nih.gov/30429277/
3. Rojo, Rocío, Raper, Anna, Ozdemir, Derya D, Hume, David A, Pridans, Clare. 2019. Deletion of a Csf1r enhancer selectively impacts CSF1R expression and development of tissue macrophage populations. In Nature communications, 10, 3215. doi:10.1038/s41467-019-11053-8. https://pubmed.ncbi.nlm.nih.gov/31324781/
4. Adams, Rachael C, Carter-Cusack, Dylan, Llanes, Genesis T, Zeiser, Robert, MacDonald, Kelli P A. . CSF1R inhibition promotes neuroinflammation and behavioral deficits during graft-versus-host disease in mice. In Blood, 143, 912-929. doi:10.1182/blood.2023022040. https://pubmed.ncbi.nlm.nih.gov/38048572/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen